Anti-YIF1A Antibody, Rabbit Polyclonal

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Anti-YIF1A Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-YIF1A Antibody, Rabbit Polyclonal
Validated applications
WB,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human YIF1A
Immunogen
E. coli-derived Human YIF1A fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human YIF1A fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-YIF1A Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
IHC-P 1:250-1:1000
IP IP:0.2-1μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-YIF1A Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-YIF1A rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:32 kDa

Observed band size:32 kDa

Immunochemical staining of human YIF1A in human gallbladder with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human YIF1A in human breast with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

YIF1A was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

1 µL anti-YIF1A rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-YIF1A rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 32 kDa

Observed band size :22 kDa

Anti-YIF1A Antibody, Rabbit Polyclonal: Synonyms

Anti-54TM Antibody; Anti-FinGER7 Antibody; Anti-YIF1 Antibody; Anti-YIF1P Antibody

YIF1A Background Information

Full Name
Yip1 interacting factor homolog A (S. cerevisiae)

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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