Anti-ZNHIT1 Antibody, Rabbit Polyclonal

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Anti-ZNHIT1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) General Information

Product name
Anti-ZNHIT1 Antibody, Rabbit Polyclonal
Validated applications
WB,ICC/IF,IP
Species reactivity
Reacts with: Human
Specificity
Human ZNHIT1
Immunogen
E. coli-derived Human ZNHIT1 fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human ZNHIT1 fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-ZNHIT1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ICC-IF 1:500-1:2500
IP IP:0.2-1μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ZNHIT1 Antibody, Rabbit Polyclonal (Rabbit Polyclonal antibody) Images

Anti-ZNHIT1 rabbit polyclonal antibody at 1:500 dilution

Lane A: U-251 MG Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:18 kDa

Observed band size:18 kDa

Immunofluorescence staining of ZNHIT1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ZNHIT1 polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

ZNHIT1 was immunoprecipitated using:

Lane A:0.5 mg U251MG Whole Cell Lysate

1 µL anti-ZNHIT1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-ZNHIT1 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size :20 kDa

Anti-ZNHIT1 Antibody, Rabbit Polyclonal: Synonyms

Anti-CG1I Antibody; Anti-ZNFN4A1 Antibody

ZNHIT1 Background Information

Full Name
zinc finger, HIT-type containing 1

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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