Anti-beta-Actin Antibody

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Anti-beta-Actin Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-beta-Actin Antibody
Validated applications
WB,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human beta-Actin
Immunogen
A synthetic peptide corresponding to the C-terminus of the human beta actin.
Preparation
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the C-terminus of the human beta actin, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS, pH7.4
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-beta-Actin Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 0.5-5 μg/ml
IHC-P 0.5-5 μg/mL
IP 1-4 uL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-beta-Actin Antibody (Rabbit Polyclonal antibody) Images

Anti-ADK mouse monoclonal antibody (Cat: ab86607) at 1 µg/ml

Lane A: RAW264.7 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: NIH/3T3 Whole Cell Lysate

Lysates/proteins at 30 µg per lane.

Secondary

Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 74 kDa

Immunochemical staining of human β-Actin in human hepatoma with rabbit polyclonal antibody (3 µg/mL, formalin-fixed paraffin embedded sections).

Anti-beta-Actin Antibody: Alternative Names

Anti-BRWS1 Antibody; Anti-PS1TP5BP1 Antibody

beta-Actin Background Information

ACTB (Actin Beta) is a Protein Coding gene. Diseases associated with ACTB include Dystonia, Juvenile-Onset and Baraitser-Winter Syndrome 1. Among its related pathways are Salivary secretion and Development Slit-Robo signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is ACTG1. TUBB, HPRT and ACTB were the most stably expressed genes for all analysis groups across meningioma and non-pathological meningeal tissue combined. ACTB, a significant upregulated gene in abdominal aortic aneurysm samples, could be regulated by CLIC4, which was significantly enriched in cell motions. Dystonia-deafness syndrome is a well-known clinical entity, with sensorineural deafness typically manifesting earlier than dystonia. ACTB p.Arg183Trp heterozygosity has been reported in six patients to cause combined infant-onset deafness and dystonia manifesting in adolescence or young adulthood.
Full Name
actin, beta
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