Sinofection® transfection
Sinofection® is a superior cationic polymer-based transfection reagent. Compared with liposomes on the market, Sinofection exhibits lower cytotoxicity, higher transfection efficiency and higher repeatability.
Sinofection® Transfection Reagent
STF02: Superior cationic polymer-based; For DNA transfection, hEK293 cell and other cells' transfection
Sinofection® Transfection Reagent Feature
| • For DNA transfection | • For eukaryotic cells like HEK293 | • For both adherent and suspension cells | • For operations with or without serum |
| • Inorganic reagent without animal ingredients | • More then 180000 L application | • Easy operation and less time taken | • Stable with strict quality control |
| • Higher transfection efficiency | • Lower cytotoxicity | • higher protein expression | • Suit for kinds of cells |
High protein expression and low cytotoxicity(EGFP protein expression transfected Sinofection ）
| 293E | |
 |
 |
| 293FT | |
 |
 |
| COS7 | |
 |
 |
| HepG2 | |
 |
 |
| BHK21 | |
 |
 |
| MCF7 | |
 |
 |
| SW480 | |
 |
 |
| CHO-DG44 | |
 |
 |
Sinofection® Transfection Reagent Suitable for Following Cell Types
Usage of Sinofection in different cell lines（96-well plate）
| Cell type | Culture medium | Cells per well | DNA | Sinofection | Medium change after 4-6h |
| 293H | DMEM | 3×104 | 0.2µg | 0.5µL | DMEM+10%FBS |
| 293FT | DMEM | 3×104 | 0.2µg | 0.5µL | DMEM+10%FBS |
| 293E | DMEM | 3×104 | 0.2µg | 0.5µL | DMEM+10%FBS |
| 293F | DMEM | 3×104 | 0.2µg | 0.5µL | DMEM+10%FBS |
| COS7 | DMEM | 1.5×104 | 0.4µg | 0.5µL | DMEM+10%FBS |
| hela | DMEM | 2×104 | 0.3µg | 0.5µL | 1640+15%FBS |
| Caco2 | MEM | 3.5×104 | 0.3µg | 0.75µL | MEM+10%FBS |
| BHK21 | MEM | 2×104 | 0.2µg | 0.5µL | MEM+10%FBS |
| CHO-DG44 | DMEM+HT+pro | 2×104 | 0.5µg | 0.5µL | DMEM+HT+pro +10%FBS |
| RAW264.7 | DMEM | 3×104 | 0.2µg | 0.5µL | DMEM +10%FBS |
| MCF7 | MEM/NEAA+0.01mg/mL insulin + sodium pyruvat | 2×104 | 0.1µg | 0.25µL | MEM/NEAA+0.01mg/mL insulin + sodium pyruvat+10%FBS |
| SW480 | IMDM | 3×104 | 0.4µg | 0.5µL | IMDM +10%FBS |
| MDCK | DMEM | 4×104 | 0.6µg | 1µL | DMEM+10%FBS |
| CHO-K1 | IMDM+Pro | 3×104 | 0.2µg | 0.5µL | IMDM+Pro +10%FBS |
| HepG2 | DMEM | 3×104 | 0.5µg | 0.75µL | DMEM+10%FBS |
| A549 | DMEM | 2×104 | 0.3µg | 0.5µL | DMEM+10%FBS |
| NIH/3T3 | DMEM | 1.5×104 | 0.1µg | 0.75µL | DMEM+10%FBS |
| vero | DMEM | 3×104 | 0.3µg | 0.75µL | DMEM+10%FBS |
| sf9 | SIM SF | 5×104 | 0.4µg | 0.75µL | SIM SF+10%FBS |
