Sinofection® transfection

Sinofection® is a superior cationic polymer-based transfection reagent. Compared with liposomes on the market, Sinofection exhibits lower cytotoxicity, higher transfection efficiency and higher repeatability.

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Sinofection® Transfection Reagent

STF02: Superior cationic polymer-based; For DNA transfection, hEK293 cell and other cells' transfection

Sinofection® Transfection Reagent Feature

• For DNA transfection • For eukaryotic cells like HEK293 • For both adherent and suspension cells • For operations with or without serum
• Inorganic reagent without animal ingredients • More then 180000 L application • Easy operation and less time taken • Stable with strict quality control
• Higher transfection efficiency • Lower cytotoxicity • higher protein expression • Suit for kinds of cells

High protein expression and low cytotoxicity(EGFP protein expression transfected Sinofection ）

293E
293FT
COS7
HepG2
BHK21
MCF7
SW480
CHO-DG44

Sinofection® Transfection Reagent Suitable for Following Cell Types

• HEK293H • MCF7 • Hela • A549
• HEK293FT • SW480 • Caco2 • NIH/3T3
• HEK293E • MDCK • BHK21 • Vero
• HEK293F • CHO-K1 • CHO-DG44 • SF9
• COS7 • HepG2 • RAW264.7  

Usage of Sinofection in different cell lines（96-well plate）

Cell type Culture medium Cells per well DNA Sinofection Medium change after 4-6h
293H DMEM 3×104 0.2µg 0.5µL DMEM + 10%FBS
293FT DMEM 3×104 0.2µg 0.5µL DMEM + 10%FBS
293E DMEM 3×104 0.2µg 0.5µL DMEM + 10%FBS
293F DMEM 3×104 0.2µg 0.5µL DMEM + 10%FBS
COS7 DMEM 1.5×104 0.4µg 0.5µL DMEM + 10%FBS
hela DMEM 2×104 0.3µg 0.5µL 1640 + 15%FBS
Caco2 MEM 3.5×104 0.3µg 0.75µL MEM + 10%FBS
BHK21 MEM 2×104 0.2µg 0.5µL MEM + 10%FBS
CHO-DG44 DMEM + HT+pro 2×104 0.5µg 0.5µL DMEM + HT + pro + 10%FBS
RAW264.7 DMEM 3×104 0.2µg 0.5µL DMEM + 10%FBS
MCF7 MEM/NEAA + 0.01mg/mL insulin + sodium pyruvat 2×104 0.1µg 0.25µL MEM/NEAA + 0.01mg/mL insulin + sodium pyruvat + 10%FBS
SW480 IMDM 3×104 0.4µg 0.5µL IMDM + 10%FBS
MDCK DMEM 4×104 0.6µg 1µL DMEM + 10%FBS
CHO-K1 IMDM+Pro 3×104 0.2µg 0.5µL IMDM + Pro + 10%FBS
HepG2 DMEM 3×104 0.5µg 0.75µL DMEM + 10%FBS
A549 DMEM 2×104 0.3µg 0.5µL DMEM + 10%FBS
NIH/3T3 DMEM 1.5×104 0.1µg 0.75µL DMEM + 10%FBS
vero DMEM 3×104 0.3µg 0.75µL DMEM + 10%FBS
sf9 SIM SF 5×104 0.4µg 0.75µL SIM SF + 10%FBS