Sinofection® is a superior cationic polymer-based transfection reagent. Compared with liposomes on the market, Sinofection exhibits lower cytotoxicity, higher transfection efficiency and higher repeatability.
STF02: Superior cationic polymer-based; For DNA transfection, hEK293 cell and other cells' transfection
• For DNA transfection | • For eukaryotic cells like HEK293 | • For both adherent and suspension cells | • For operations with or without serum |
• Inorganic reagent without animal ingredients | • More then 180000 L application | • Easy operation and less time taken | • Stable with strict quality control |
• Higher transfection efficiency | • Lower cytotoxicity | • higher protein expression | • Suit for kinds of cells |
High protein expression and low cytotoxicity(EGFP protein expression transfected Sinofection )
293E | |
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293FT | |
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COS7 | |
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HepG2 | |
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BHK21 | |
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MCF7 | |
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SW480 | |
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CHO-DG44 | |
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Cell type | Culture medium | Cells per well | DNA | Sinofection | Medium change after 4-6h |
293H | DMEM | 3×104 | 0.2µg | 0.5µL | DMEM + 10%FBS |
293FT | DMEM | 3×104 | 0.2µg | 0.5µL | DMEM + 10%FBS |
293E | DMEM | 3×104 | 0.2µg | 0.5µL | DMEM + 10%FBS |
293F | DMEM | 3×104 | 0.2µg | 0.5µL | DMEM + 10%FBS |
COS7 | DMEM | 1.5×104 | 0.4µg | 0.5µL | DMEM + 10%FBS |
hela | DMEM | 2×104 | 0.3µg | 0.5µL | 1640 + 15%FBS |
Caco2 | MEM | 3.5×104 | 0.3µg | 0.75µL | MEM + 10%FBS |
BHK21 | MEM | 2×104 | 0.2µg | 0.5µL | MEM + 10%FBS |
CHO-DG44 | DMEM + HT+pro | 2×104 | 0.5µg | 0.5µL | DMEM + HT + pro + 10%FBS |
RAW264.7 | DMEM | 3×104 | 0.2µg | 0.5µL | DMEM + 10%FBS |
MCF7 | MEM/NEAA + 0.01mg/mL insulin + sodium pyruvat | 2×104 | 0.1µg | 0.25µL | MEM/NEAA + 0.01mg/mL insulin + sodium pyruvat + 10%FBS |
SW480 | IMDM | 3×104 | 0.4µg | 0.5µL | IMDM + 10%FBS |
MDCK | DMEM | 4×104 | 0.6µg | 1µL | DMEM + 10%FBS |
CHO-K1 | IMDM+Pro | 3×104 | 0.2µg | 0.5µL | IMDM + Pro + 10%FBS |
HepG2 | DMEM | 3×104 | 0.5µg | 0.75µL | DMEM + 10%FBS |
A549 | DMEM | 2×104 | 0.3µg | 0.5µL | DMEM + 10%FBS |
NIH/3T3 | DMEM | 1.5×104 | 0.1µg | 0.75µL | DMEM + 10%FBS |
vero | DMEM | 3×104 | 0.3µg | 0.75µL | DMEM + 10%FBS |
sf9 | SIM SF | 5×104 | 0.4µg | 0.75µL | SIM SF + 10%FBS |