Anti-CD44 Antibody

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Anti-CD44 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-CD44 Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human CD44
Immunogen
Recombinant Human CD44 protein (Catalog#12211-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD44 (rh CD44; Catalog#12211-H08H; P16070-1; Met 1-Pro 220).
Source
Monoclonal Rabbit IgG Clone #065
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD44 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD44 Antibody (Rabbit Monoclonal antibody) Images

Anti-CD44 rabbit monoclonal antibody at 1:500 dilution

Lane A: HL60 Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:82 kDa

Observed band size:82 kDa

(We are unsure as to the identity of these extra bands.)

Anti-CD44 Antibody: Alternative Names

Anti-CDW44 Antibody; Anti-CSPG8 Antibody; Anti-ECMR-III Antibody; Anti-HCELL Antibody; Anti-HUTCH-I Antibody; Anti-IN Antibody; Anti-LHR Antibody; Anti-MC56 Antibody; Anti-MDU2 Antibody; Anti-MDU3 Antibody; Anti-MIC4 Antibody; Anti-Pgp1 Antibody

CD44 Background Information

CD44 is a type I transmembrane protein and a member of the cartilage link protein family. It is involved in cell-cell and cell-matrix interactions and signal transduction. Several CD44 ligands have been identified. The most extensively characterized ligand for CD44 is hyaluronan, a component of the extracellular matrix. CD44 protein is expressed on the majority of immune cells. The binding of CD44 to hyaluronan is induced on T lymphocytes after activation by antigen and on monocytes after stimulation by inflammatory agents. Under inflammatory conditions, CD44 on endothelial cells presents hyaluronan to CD44 on activated T lymphocytes and mediates a rolling interaction under flow conditions. Perturbations of the hyaluronan-CD44 interaction at the plasma membrane by various antagonists result in attenuation of receptor tyrosine kinase and transporter activities and inhibition of tumor progression in vivo. CD44 is known to interact with the ezrin family (ERM family) members and form a complex that plays diverse roles within both normal and abnormal cells, particularly cancer cells. CD44 and ezrin and their respective complex have properties suggesting that they may be important in the process of tumour-endothelium interactions, cell migrations, cell adhesion, tumour progression and metastasis.
Full Name
CD44 molecule (Indian blood group)
References
  • Bajorath J. (2000) Molecular organization, structural features, and ligand binding characteristics of CD44, a highly variable cell surface glycoprotein with multiple functions. Proteins. 39(2): 103-11.
  • Johnson P, et al. (2000) A role for the cell adhesion molecule CD44 and sulfation in leukocyte-endothelial cell adhesion during an inflammatory response? Biochem Pharmacol. 59(5): 455-65.
  • Martin TA, et al. (2003) The role of the CD44/ezrin complex in cancer metastasis. Crit Rev Oncol Hematol. 46(2): 165-86.
  • Toole BP, et al. (2008) Hyaluronan, CD44 and Emmprin: partners in cancer cell chemoresistance. Drug Resist Updat. 11(3): 110-21.
  • Johnson P, et al. (2009) CD44 and its role in inflammation and inflammatory diseases. Inflamm Allergy Drug Targets. 8(3): 208-20.
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