Anti-CD50 Antibody

1/2
Price:
Size:
Number:

Anti-CD50 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-CD50 Antibody
Validated applications
IHC-P
Species reactivity
Reacts with: Human
Specificity
Human CD50
No cross-reactivity in ELISA with
Mouse ICAM1
Human ICAM
Human VCAM1
Mouse VCAM1
Immunogen
Recombinant Human CD50 / ICAM-3 protein (Catalog#10333-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD50 / ICAM-3 (rh CD50 / ICAM-3; Catalog#10333-H08H; NP_002153.2; Met1-His485).
Source
Monoclonal Rabbit IgG Clone #002
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD50 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
IHC-P 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD50 Antibody (Rabbit Monoclonal antibody) Images

Immunochemical staining of human ICAM3 in human tonsil with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).
Immunochemical staining of human ICAM3 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).

Anti-CD50 Antibody: Alternative Names

Anti-CD50 Antibody; Anti-CDW50 Antibody; Anti-ICAM-3 Antibody; Anti-ICAM-R Antibody

CD50 Background Information

The protein ICAM-3, also known as CD5, is a member of the intercellular adhesion molecule (ICAM) family consisting three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes, and is thus an important molecule for the first immune response. ICAM-3 comprises of five immunoglobulin-like domains, and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that CD5 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. In addition, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase of cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker.
Full Name
intercellular adhesion molecule 3
References
  • Berney SM, et al. (1999) ICAM-3 (CD50) cross-linking augments signaling in CD3-activated peripheral human T lymphocytes. J Leukoc Biol. 65(6): 867-74.
  • van Buul JD, et al. (2004) ICAM-3 activation modulates cell-cell contacts of human bone marrow endothelial cells. J Vasc Res. 41(1): 28-37.
  • Sugino H. (2005) ICAM-3, a ligand for DC-SIGN, was duplicated from ICAM-1 in mammalian evolution, but was lost in the rodent genome. FEBS Lett. 579(13): 2901-6.
  • Park JK, et al. (2010) ICAM-3 enhances the migratory and invasive potential of human non-small cell lung cancer cells by inducing MMP-2 and MMP-9 via Akt and CREB. Int J Oncol. 36(1): 181-92.
  • Estradiol Promotes Breast Cancer Cell Migration via Recruitment and Activation of Neutrophils
    Author
    Vazquez Rodriguez, G;Abrahamsson, A;Jensen, LD;Dabrosin, C;
    Year
    2017
    Journal
    Cancer Immunol Res
    Application
    IHC

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait