CD64 Protein, Human, Recombinant (His Tag)

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CD64 Protein, Human, Recombinant (His Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its binding ability in a functional ELISA
.
1. Immobilized CD64 at 10 μg/ml can bind human IgG1 with a linear range of 0.39-25 ng/ml.
2. Scatchard analysis showed the affinity constant (Kd) of CD64 bound to Human IgG1 was 0.059 nM
.
Protein Construction
A DNA sequence encoding the extracellular domain of human CD64A (NP_000557.1) (Met 1-Pro 288) was expressed, fused with a polyhistidine tag at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gln 16
Molecule Mass
The recombinant human CD64A consists of 284 amino acids and has a predicted molecular mass of 32 kDa.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD64 Protein, Human, Recombinant (His Tag): Images

CD64 Protein, Human, Recombinant (His Tag): Alternative Names

CD64 Protein, Human; Fc gamma RI Protein, Human

CD64 Background Information

High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.
Full Name
Fc receptor, IgG, high affinity I
References
  • Allen J.M., et al.,(1988), Nucleotide sequence of three cDNAs for the human high affinity Fc receptor (FcRI). Nucleic Acids Res. 16:11824-11824.
  • Allen J.M., et al., (1989), Isolation and expression of functional high-affinity Fc receptor complementary DNAs.Science 243:378-381.
  • Porges A.J., et al.,(1992), Novel Fc gamma receptor I family gene products in human mononuclear cells.J. Clin. Invest. 90:2102-2109.
  • Bispecific igg antibodies as t cell engagers
    Author
    ABH Bakker, PF Van Loo…
    Year
    2013
    Journal
    US 20140120096 A1
  • Fc Gamma Receptor Glycosylation Modulates the Binding of IgG Glycoforms: A Requirement for Stable Antibody Interactions
    Author
    Hayes, JM;Frostell, A;Cosgrave, EF;Struwe, WB;Potter, O;Davey, GP;Karlsson, R;Anneren, C;Rudd, PM;
    Year
    2014
    Journal
    J. Proteome Res.
    Application
    glycosylation analysis and binding
  • Enhanced Effector Functions Due to Antibody Defucosylation Depend on the Effector Cell Fcγ Receptor Profile
    Author
    Bruggeman, CW;Dekkers, G;Bentlage, AEH;Treffers, LW;Nagelkerke, SQ;Lissenberg-Thunnissen, S;Koeleman, CAM;Wuhrer, M;van den Berg, TK;Rispens, T;Vidarsson, G;Kuijpers, TW;
    Year
    2017
    Journal
    J. Immunol.
    Application
    SPR
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