Chikungunya virus (CHIKV) (strain SL-CK1) E2 glycoprotein Gene ORF cDNA clone expression plasmid, C-HA tag

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Chikungunya virus (CHIKV) (strain SL-CK1) E2 glycoprotein Gene ORF cDNA clone expression plasmid, C-HA tag: General Information

Gene
Species
CHIKV
RefSeq ORF Size
1275 bp
Sequence Description
A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with HM045801.1.
Description
Full length Clone DNA of Chikungunya virus (CHIKV), strain SL-CK1 glycoprotein E2 with C terminal HA tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
Tag Sequence
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

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