Influenza B (B/Florida/4/2006) Nucleocapsid protein / NP (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-His tag
Cat: VG40438-CH
Influenza B (B/Florida/4/2006) Nucleocapsid protein / NP (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-His tag: General Information
Gene
Species
Influenza B
RefSeq ORF Size
1728 bp
Sequence Description
A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with ACF54251.1.
Description
Full length Clone DNA of Influenza B (B/Florida/4/2006) Nucleocapsid protein with C terminal His tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
Restriction Sites
KpnI + XbaI(6kb+1.73kb)
Tag Sequence
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
Nucleocapsid/NP cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
Influenza B (B/Florida/4/2006) Nucleocapsid protein / NP (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-His tag: Validated Images
Influenza B (B/Florida/4/2006) Nucleocapsid protein / NP (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-His tag: Synonyms
NP cDNA ORF Clone, Influenza B; Nucleoprotein cDNA ORF Clone, Influenza B
Nucleocapsid/NP Background Information
Influenza A viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation. The influenza A virus nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs from all strains of influenza A viruses contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. The nucleocapsid is a complex of the viral nucleoprotein, RNA, and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins.
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