Anti-Cyclin E Antibody

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Anti-Cyclin E Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-Cyclin E Antibody
Validated applications
WB,ELISA,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human Cyclin E
Immunogen
Recombinant Human CCNE1 protein (Catalog#10902-H07B)
Preparation
Produced in rabbits immunized with purified, recombinant Human CCNE1 (rh CCNE1 , Catalog#10902-H07B; NP_001229.1; Met 1-Ala 410). CCNE1 specific IgG was purified by human CCNE1 affinity chromatography .
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Cyclin E Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:25000-1:50000
IHC-P 1:2000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Cyclin E Antibody (Rabbit Polyclonal antibody) Images

Immunochemical staining of human CCNE1 in human placenta with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. The image showing nucleus staining of decidual cells. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein.

Anti-CCNE1 rabbit polyclonal antibody at 1:500 dilution

Lane A: U87-MG Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:47 kDa

Observed band size:47 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Cyclin E Antibody: Alternative Names

Anti-CCNE Antibody

Cyclin E Background Information

Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.
Full Name
cyclin E1
References
  • Honda R, et al. (2005) The structure of cyclin E1 / CDK2: implications for CDK2 activation and CDK2-independent roles. The EMBO Journal. 24: 452-63.
  • Geng Y, et al. (2007) Kinase-Independent Function of Cyclin E. 25(1): 127-39.
  • Impacts of CyhospitalclinE downstream vimentin on proliferation, invasion and apoptosis of hepatoma HepG2 cell
    Author
    Hu, G;Jia, F;Gao, N;Han, Y;
    Year
    2018
    Journal
    International Journal of Clinical and Experimental Medicine
    Application
    WB

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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