CD21 ELISA Kit, Human

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CD21 ELISA Kit, Human: General Information

Product name
CD21 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
8.75 pg/mL
Assay range
39.06-2500 pg/mL
Specificity
Recognizes both recombinant and natural Human CD21
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 561.00 1,035.00 1,979.00
SD 8.42 15.48 29.28
CV(%) 1.5% 1.5% 1.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 377.00 796.00 1,523.00
SD 19.30 69.73 87.97
CV(%) 5.1% 8.8% 5.8%
Recovery
The recovery of Human CR2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
90
Range
82 -106%
Sample
supernatant (n=3)
Average % Recovery
113
Range
108 -117%
Linearity
Serum
  Recovery of detected
1:2 100%
1:4 100%
1:8 82%
1:16 87%
Supernatant
  Recovery of detected
1:2 101%
1:4 99%
1:8 92%
1:16 85%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This CD21 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD21 protein in Serum . It contains recombinant Human CD21, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD21 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CD21. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CD21 ELISA Kit, Human: Synonyms

C3DR ELISA Kit, Human; CD21 ELISA Kit, Human; CR ELISA Kit, Human; CVID7 ELISA Kit, Human; SLEB9 ELISA Kit, Human

CD21 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD21, also known as Complement component (3d / Epstein Barr virus) receptor 2 and CR2, is a member of the CD system and is a protein involved in complement system. CD21 is present on all mature B-cells and some T-cells and follicular dendritic cells. CD21 on mature B-cells form a complex called the B cell receptor complex with two other membrane proteins, CD19 and CD81. CD21 has a function in the complement system through serving as the cellular receper specific for ligands such as C3 and C4 which can be attached to foreign macromolecules in order to remove or uptake them. This results in B-cells having enhanced response to the antigen.
Full Name
complement component (3d/Epstein Barr virus) receptor 2
Related Pathways
  • Complement Activation Pathways
    Complement Activation Pathways
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Joseph M, et al. (1989) Structure and Function of the Complement Receptors, CR1 (CD35) and CR2 (CD21). Advanced in immunology. 46: 183-219.
  • Aubry JP, et al. (1992) CD21 is a ligand for CD23 and regulates IgE production. Nature 358: 505 - 7.
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