Fractalkine ELISA Kit, Human

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Fractalkine ELISA Kit, Human: General Information

Product name
Fractalkine ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Milk
Sensitivity
2.96 pg/mL
Assay range
7.82-500 pg/mL
Specificity
Recognizes both recombinant and natural Human Fractalkine
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 64.00 168.00 317.00
SD 6.00 9.49 14.77
CV(%) 9.3% 5.6% 4.4%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 103.00 214.00 422.00
SD 9.66 17.81 33.09
CV(%) 9.4% 8.3% 7.8%
Recovery
The recovery of Human CX3CL1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
milk (n=3)
Average % Recovery
73
Range
72 -74%
Linearity
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This Fractalkine ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Fractalkine protein in Milk . It contains recombinant Human Fractalkine, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Fractalkine ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CX3CL1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Citation List

Fractalkine ELISA Kit, Human: Synonyms

ABCD-3 ELISA Kit, Human; C3Xkine ELISA Kit, Human; CXC3 ELISA Kit, Human; CXC3C ELISA Kit, Human; fractalkine ELISA Kit, Human; neurotactin ELISA Kit, Human; NTN ELISA Kit, Human; NTT ELISA Kit, Human; SCYD1 ELISA Kit, Human

Fractalkine Background Information

Fractalkine or Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a member of the CX3C chemokine family. Fractalkine / CX3CL1 is a unique chemokine that functions not only as a chemoattractant but also as an adhesion molecule and is expressed on endothelial cells activated by proinflammatory cytokines, such as interferon-gamma and tumor necrosis factor-alpha. Fractalkine/CX3CL1 is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Fractalkine / CX3CL1 is associated with dendritic cells (DC) in epidermis and lymphoid organs. The fractalkine receptor, CX3CR1, is expressed on cytotoxic effector lymphocytes, including natural killer (NK) cells and cytotoxic T lymphocytes, which contain high levels of intracellular perforin and granzyme B, and on macrophages. Soluble fractalkine causes migration of NK cells, cytotoxic T lymphocytes, and macrophages, whereas the membrane-bound form captures and enhances the subsequent migration of these cells in response to secondary stimulation with other chemokines.
Full Name
chemokine (C-X3-C motif) ligand 1
Research Areas
References
  • Imai T, et al. (1997) Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell. 91(4): 521-30.
  • Papadopoulos EJ, et al. (1999) Fractalkine, a CX3C chemokine, is expressed by dendritic cells and is up-regulated upon dendritic cell maturation. Eur J Immunol. 29 (8): 2551-9.
  • Umehara H, et al. (2004) Fractalkine in vascular biology: from basic research to clinical disease". Arterioscler. Thromb Vasc Biol. 24 (1): 34-40.
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