M-CSF ELISA Kit, Human

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M-CSF ELISA Kit, Human: General Information

Product name
M-CSF ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum,Cell supernatant,Urine
Sensitivity
11.72 pg/mL
Assay range
11.72-750 pg/mL
Specificity
Recognizes both recombinant and natural Human M-CSF
Interference
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human MCSF control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 198.59 429.24 895.16
SD 6.65 25.50 35.37
CV(%) 3.3% 5.9% 4.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 203.54 431.22 852.14
SD 6.24 15.67 23.49
CV(%) 3.1% 3.6% 2.8%
Recovery
The recovery of Human CSF1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
82
Range
73-88%
Sample
supernatant (n=3)
Average % Recovery
105
Range
94-113%
Sample
urine (n=3)
Average % Recovery
92
Range
86-96%
Linearity
Serum
  Recovery of detected
1:2 99%
1:4 101%
1:8 101%
1:16 86%
Supernatant
  Recovery of detected
1:2 103%
1:4 97%
1:8 91%
1:16 77%
Urine
  Recovery of detected
1:2 102%
1:4 98%
1:8 89%
1:16 73%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This M-CSF ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human M-CSF protein in Serum,Cell supernatant,Urine . It contains recombinant Human M-CSF, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

M-CSF ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human MCSF. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Citation List

M-CSF ELISA Kit, Human: Synonyms

CSF-1 ELISA Kit, Human; M-CSF ELISA Kit, Human; MCSF ELISA Kit, Human

M-CSF Background Information

Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.
Full Name
colony stimulating factor 1 (macrophage)
References
    1. Pandit J. et al., 1992, Science. 258: 1358-62.
    2. Tokai M. et al., 2000, J Bacteriol. 182 (10): 2865-8.
    3. Fan X. et al., 2001, Am J Physiol Endocrinol Metab. 280 (1): E103-11.
    4. Frangogiannis NG. et al., 2003, Am J Physiol Heart Circ Physiol. 285 (2): H483-92.
    5. Cupp JS. et al., 2007, Am J Surg Pathol. 31 (6): 970-6.
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