The kit is used for detection and quantitative determination of SuperNuclease and Benzonase® endonuclease, and also can detect the denatured SuperNuclease and Benzonase® .
Note: Benzonase® (Benzonase is a trademark of Merck KGaA, Darmstadt, Germany).
The principle of this ELISA kit is based on the solid phase sandwich enzyme immunoassay technique. A monoclonal antibody specific for SuperNuclease has been pre-coated onto well plate strips. Standards and samples are added to the wells and SuperNuclease present in the sample is bound by the immobilized antibody. After incubation the wells are washed and a horseradish peroxidase conjugated anti-SuperNuclease antibody is added, producing an antibody-antigen-antibody "sandwich complex". Following a wash to remove any unbound antibody a TMB substrate solution is loaded and color develops in proportion to the amount of SuperNuclease bound. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm (See schematics below).
|Components||1 Kit (96 Tests)|
|Microplate: Pre-coated with anti-SuperNuclease mouse monoclonal antibody||1 Plate|
|Detection Antibody: Anti-SuperNuclease rabbit monoclonal antibody (HRP)||40 μL|
|Standard: SuperNuclease (Cat: GMP-SSNP01)||1 vial|
|20 × Dilution Buffer||8 mL|
|20 × Wash Buffer||25 mL|
|Color Reagent A||13 mL|
|Color Reagent B||13 mL|
|Stop Solution||8 mL|
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA