Anti-Endoglin / CD105 Antibody

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Anti-Endoglin / CD105 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-Endoglin / CD105 Antibody
Validated applications
WB,ELISA,FCM
Species reactivity
Reacts with: Mouse
Specificity
Mouse Endoglin / CD105
No cross-reactivity in ELISA with
Human CD105
Immunogen
Recombinant Mouse Endoglin / CD105 / ENG Protein (Catalog#50407-M08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Endoglin / CD105 / ENG (rM Endoglin / CD105 / ENG; Catalog#50407-M08H; NP_031958.2; Met 1-Gly 580).
Source
Monoclonal Rabbit IgG Clone #001
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Endoglin / CD105 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:25000-1:50000
FCM 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Endoglin / CD105 Antibody (Rabbit Monoclonal antibody) Images

Flow cytometric analysis of Mouse ENG(CD105) expression on bEnd.3 cells. Cells were stained with purified anti-Mouse ENG(CD105), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-ENG rabbit monoclonal antibody at 1:500 dilution

Lane A: MCF7 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:70 kDa

Observed band size:45 kDa

Anti-Endoglin / CD105 Antibody: Alternative Names

Anti-AI528660 Antibody; Anti-AI662476 Antibody; Anti-CD105 Antibody; Anti-Endo Antibody; Anti-S-endoglin Antibody

Endoglin / CD105 Background Information

Endoglin, also known as CD15, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type II receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.
Full Name
endoglin
References
  • Bellon T., et al.,(1993), Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions. Eur. J. Immunol. 23:2340-2345.
  • Humphray S.J., et al., (2004), DNA sequence and analysis of human chromosome 9.Nature 429:369-374.
  • Gougos A., et al.,(1990), Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells.J. Biol. Chem. 265:8361-8364.

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Mouse MAb

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Mouse MAb

Rabbit PAb

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