Nectin-2 ELISA Kit, Human

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Nectin-2 ELISA Kit, Human: General Information

Product name
Nectin-2 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
7.95 pg/mL
Assay range
23.4-1500 pg/mL
Specificity
Recognizes both recombinant and natural Human Nectin-2
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 230.57 549.40 913.97
SD 8.33 18.79 31.20
CV(%) 3.6% 3.4% 3.4%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 254.20 582.20 942.92
SD 17.14 18.74 91.60
CV(%) 6.7% 3.2% 9.7%
Recovery
The recovery of Human NECTIN2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=2)
Average % Recovery
84
Range
81 -87%
Linearity
Serum
  Recovery of detected
1:2 90%
1:4 94%
1:8 106%
1:16 92%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This Nectin-2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Nectin-2 protein in Serum . It contains recombinant Human Nectin-2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Nectin-2 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Nectin-2 ELISA Kit, Human: Alternative Names

CD112 ELISA Kit, Human; HVEB ELISA Kit, Human; Nectin-2 ELISA Kit, Human; PRR2 ELISA Kit, Human; PVRR2 ELISA Kit, Human

Nectin-2 Background Information

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
Full Name
poliovirus receptor-related 2 (herpesvirus entry mediator B)
References
  • Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.
  • Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
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