TNF beta ELISA Kit, Human

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TNF beta ELISA Kit, Human: General Information

Product name
TNF beta ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Cell supernatant
Sensitivity
3.13 pg/mL
Assay range
7.82-500 pg/mL
Specificity
Recognizes both recombinant and natural Human TNF beta
Interference
Preparations of the following factors at 50 ng/mL in a mid-range recombinant Human TNFβ control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 75.00 162.00 305.00
SD 2.82 4.53 12.30
CV(%) 3.8% 2.8% 4.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 84.00 164.00 332.00
SD 6.35 13.61 20.60
CV(%) 7.6% 8.3% 6.2%
Recovery
The recovery of Human LTA spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
84
Range
81 -87%
Sample
supernatant (n=3)
Average % Recovery
113
Range
112 -113%
Sample
urine (n=3)
Average % Recovery
84
Range
81 -87%
Linearity
Supernatant
  Recovery of detected
1:2 93%
1:4 98%
1:8 109%
1:16 95%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This TNF beta ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human TNF beta protein in Cell supernatant . It contains recombinant Human TNF beta, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

TNF beta ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human TNFβ. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

TNF beta ELISA Kit, Human: Alternative Names

LT ELISA Kit, Human; TNFB ELISA Kit, Human; TNFSF1 ELISA Kit, Human

TNF beta Background Information

Lymphotoxin-alpha, also known as LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA TNFSF1 and TNFB, is a secreted protein which belongs to the tumor necrosis factor family. TNF-beta/TNFSF1/Lymphotoxin alpha is a highly inducible, secreted, and exists as homotrimeric molecule. It is a cytokine that in its homotrimeric form binds to TNFRSF1A / TNFR1, TNFRSF1B / TNFBR and TNFRSF14 / HVEM. In its heterotrimeric form with LTB, TNF-beta/TNFSF1/Lymphotoxin alpha binds to TNFRSF3 / LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells. TNF-beta/TNFSF1/Lymphotoxin alpha forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. It mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. TNF-beta/TNFSF1/Lymphotoxin alpha is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in TNF-beta/TNFSF1/Lymphotoxin alpha are a cause of susceptibility psoriatic arthritis which is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy.
Full Name
lymphotoxin alpha
References
  • Messer G, et al. (1991) Polymorphic structure of the tumor necrosis factor (TNF) locus: an NcoI polymorphism in the first intron of the human TNF-beta gene correlates with a variant amino acid in position 26 and a reduced level of TNF-beta production. J Exp Med. 173(1): 209-19.
  • Banner DW, et al. (1993) Crystal structure of the soluble human 55 kd TNF receptor-human TNF beta complex: implications for TNF receptor activation. Cell. 73(3): 431-45.
  • Picarella DE, et al. (1993) Transgenic tumor necrosis factor (TNF)-alpha production in pancreatic islets leads to insulitis, not diabetes. Distinct patterns of inflammation in TNF-alpha and TNF-beta transgenic mice. J Immunol. 150(9): 4136-50.
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