Anti-Carboxypeptidase A Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-Carboxypeptidase A Magnetic Beads-IP Kit Product Components

Components Storage
Anti-Carboxypeptidase A Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-Carboxypeptidase A magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-Carboxypeptidase A Magnetic Beads-IP Kit Product Description

The Anti-Carboxypeptidase A magnetic Beads, conjugated with Anti-Carboxypeptidase A antibody, are used for immuneprecipitation (IP) of Carboxypeptidase A proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Carboxypeptidase A proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Carboxypeptidase A proteins are dissociated from the magnetic beads using an elution buffer.

Anti-Carboxypeptidase A Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-Carboxypeptidase A Antibody(10504-RP02)
Immunogen
Recombinant Human Carboxypeptidase A1 / CPA1 protein (Catalog#10504-H08H)
Species Reactivity
Human Carboxypeptidase A1 / CPA1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Carboxypeptidase A1 / CPA1 (rh Carboxypeptidase A1 / CPA1; Catalog#10504-H08H; NP_001859.1; Met 1-Tyr 419). Carboxypeptidase A1 / CPA1 specific IgG was purified by Human Carboxypeptidase A1 / CPA1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-Carboxypeptidase A Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-CPAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

Carboxypeptidase A Background Information

Carboxypeptidase A1 (CPA1)is secreted as a pancreatic procarboxypeptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated.
Full Name
carboxypeptidase A1 (pancreatic)
References
  • Catasus, L. et al., 1992, Biochem. J. 287: 299-303.
  • Moulard, M. et al., 1990, FEBS. Lett. 261: 179-183.
  • Aloy, P. et al., 1998, Biol. Chem. 379: 149-155.
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