Anti-Nectin-2 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-Nectin-2 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-Nectin-2 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-Nectin-2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-Nectin-2 Magnetic Beads-IP Kit Product Description

The Anti-Nectin-2 magnetic Beads, conjugated with Anti-Nectin-2 antibody, are used for immuneprecipitation (IP) of Nectin-2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Nectin-2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Nectin-2 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-Nectin-2 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-Nectin-2 Antibody(10005-RP04)
Immunogen
Recombinant Human CD112 / Nectin2 protein (Catalog#10005-H08H)
Species Reactivity
Human CD112 / Nectin2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CD112 / Nectin-2 extracellular domain (rhCD112; Catalog#10005-H08H; Met 1-Leu 360; NP_002847.1). CD112 specific IgG was purified by human CD112 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-Nectin-2 Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-CD112ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-HVEBALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Nectin-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PRR2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PVRR2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

Nectin-2 Background Information

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
Full Name
poliovirus receptor-related 2 (herpesvirus entry mediator B)
References
  • Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.
  • Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
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