Anti-Nectin-2 Magnetic Beads Immunoprecipitation (IP) Kit


Anti-Nectin-2 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-Nectin-2 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-Nectin-2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-Nectin-2 Magnetic Beads-IP Kit Product Description

The Anti-Nectin-2 magnetic Beads, conjugated with Anti-Nectin-2 antibody, are used for immuneprecipitation (IP) of Nectin-2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Nectin-2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Nectin-2 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-Nectin-2 Magnetic Beads-IP Kit Antibody Information

Anti-Nectin-2 Antibody(50318-R110)
Recombinant Mouse PVRL2 protein (Catalog#50318-M08H)
Species Reactivity
Mouse PVRL2 / CD112 / Nectin-2
Monoclonal Mouse Rabbit IgG
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse PVRL2 extracellular domain (rM PVRL2; Catalog#50318-M08H; NP_033016.3; Met 1-Gly 351).
Immunoprecipitation (IP), Minimum Protein Purification

Anti-Nectin-2 Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-AI325026ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-AI987993ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Cd112ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MPHALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-nectin-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PvrALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PvsALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

Nectin-2 Background Information

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
Full Name
poliovirus receptor-related 2 (herpesvirus entry mediator B)
  • Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.
  • Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
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