Anti-BTK Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-BTK Magnetic Beads-IP Kit Product Components

Components Storage
Anti-BTK Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-BTK magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-BTK Magnetic Beads-IP Kit Product Description

The Anti-BTK magnetic Beads, conjugated with Anti-BTK antibody, are used for immuneprecipitation (IP) of BTK proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing BTK proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound BTK proteins are dissociated from the magnetic beads using an elution buffer.

Anti-BTK Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-BTK Antibody(10578-T44)
Immunogen
Recombinant Human Bruton Tyrosine Kinase / BTK Kinase protein (Catalog#10578-H07B)
Species Reactivity
Human Bruton Tyrosine Kinase / BTK Kinase
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Bruton Tyrosine Kinase / BTK Kinase (rh Bruton Tyrosine Kinase / BTK Kinase; Catalog#10578-H07B; NP_000052.1; Met1-Ser659). Bruton Tyrosine Kinase / BTK Kinase specific IgG was purified by Human Bruton Tyrosine Kinase / BTK Kinase affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-BTK Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-AGMX1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ATALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ATKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-BPKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-IMD1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PSCTK1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-XLAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

BTK Background Information

Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3,4,5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.
Full Name
Bruton agammaglobulinemia tyrosine kinase
Research Areas
Related Pathways
  • NF-kB (NFkB) Pathway
    NF-kB (NFkB) Pathway
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
References
  • Hashimoto S, et al. (1996) Identification of Bruton's tyrosine kinase (Btk) gene mutations and characterization of the derived proteins in 35 X-linked agammaglobulinemia families: a nationwide study of Btk deficiency in Japan. Blood. 88(2): 561-73.
  • Ohta Y, et al. (1994) Genomic organization and structure of Bruton agammaglobulinemia tyrosine kinase: localization of mutations associated with varied clinical presentations and course in X chromosome-linked agammaglobulinemia. PNAS. 91(19): 9062-6.
  • Smith C, et al. (1994) Expression of Bruton's agammaglobulinemia tyrosine kinase gene, BTK, is selectively down-regulated in T lymphocytes and plasma cells. The Journal of Immunology. 152(2): 557-65.
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