Anti-CAMKV Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CAMKV Magnetic Beads-IP Kit Product Components

Components Storage
Anti-CAMKV Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CAMKV magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CAMKV Magnetic Beads-IP Kit Product Description

The Anti-CAMKV magnetic Beads, conjugated with Anti-CAMKV antibody, are used for immuneprecipitation (IP) of CAMKV proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CAMKV proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CAMKV proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CAMKV Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-CAMKV Antibody(200989-T38)
Immunogen
E. coli-derived Human CAMKV fragment
Species Reactivity
Human CAMKV
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with E. coli-derived Human CAMKV fragment, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CAMKV Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-1G5ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-VACAMKLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CAMKV Background Information

CaM kinase-like vesicle-associated protein, also known as CAMKV, is a peripheral membrane protein and Cytoplasmic vesicle membrane protein which belongs to theprotein kinase superfamily and CAMK Ser/Thr protein kinase family. CAMKV contains oneprotein kinase domain. It is predominantly observed in association with the plasma membrane of soma and in neurites, both axons and dendrites. CAMKV may be associated with vesicular structures. It does not appear to have detectable kinase activity. Protein kinases are a group of enzymes that move a phosphate group onto proteins, in a process called phosphorylation. Protein kinases function as an on/off switch for many cellular processes, including metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. They also function in embryonic development, physiological responses, and in the nervous and immune system. Abnormal phosphorylation causes many human diseases, including cancer, and drugs that affect phosphorylation can treat those diseases. The protein kinase domain is a structurally conserved protein domain containing the catalytic function of protein kinases. Protein kinases play a role in a mulititude of cellular processes, including division, proliferation, apoptosis, and differentiation. Phosphorylation usually results in a functional change of the target protein by changing enzyme activity, cellular location, or association with other proteins.
Full Name
CaM kinase-like vesicle-associated
Related Pathways
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References
  • Hunter T, et al.,1988, Science. 241 (4861): 42-51.
  • Wiemann S., et al., 2001, Genome Res. 11:422-435.
  • G. Manning, et al., 2002, Science 6. 298:1912-1934.
  • Manning G, et al.,2002, Science. 298 (5600): 1912-34.
  • Ota T., et al., 2004, Nat. Genet. 36:40-45.
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