Anti-IGFBP7 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-IGFBP7 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-IGFBP7 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-IGFBP7 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-IGFBP7 Magnetic Beads-IP Kit Product Description

The Anti-IGFBP7 magnetic Beads, conjugated with Anti-IGFBP7 antibody, are used for immuneprecipitation (IP) of IGFBP7 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing IGFBP7 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound IGFBP7 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-IGFBP7 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-IGFBP7 Antibody, Rabbit Polyclonal(13100-T52)
Immunogen
Recombinant Human IGFBP7 Protein (Catalog#13100-H08H)
Species Reactivity
Human IGFBP7
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human IGFBP7 (rh IGFBP7; Catalog#13100-H08H; Q16270; Met1-Leu282). IGFBP7 specific IgG was purified by Human IGFBP7 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-IGFBP7 Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-AGMALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-FSTL2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-IBP-7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-IGFBP-7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-IGFBP-7vALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-IGFBPRP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MAC25ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PSFALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-RAMSVPSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TAFALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

IGFBP7 Background Information

Insulin-like growth factor-binding protein 7 (IGFBP7) is a member of the IGFBP family. It has been identified in colorectal adenocarcinoma (CRC) cell lines. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF or act in an IGF-independent manner in the prostate. IGFBP7 could inhibit cell growth, decrease soft agar colony formation activity, and induce apoptosis in RKO and SW620 cells. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence, and IGF affinity.
Full Name
insulin-like growth factor binding protein 7
References
  • Oh Y, et al. (1996) Synthesis and characterization of insulin-like growth factor-binding protein (IGFBP)-7. Recombinant human mac25 protein specifically binds IGF-I and -II. J Biol Chem. 271(48): 30322-5.
  • Wilson EM, et al. (1997) Generation and characterization of an IGFBP-7 antibody: identification of 31kD IGFBP-7 in human biological fluids and Hs578T human breast cancer conditioned media. J Clin Endocrinol Metab. 82(4): 1301-3.
  • Lin J, et al. (2007) Methylation patterns of IGFBP7 in colon cancer cell lines are associated with levels of gene expression. J Pathol. 212(1): 83-90.
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