Anti-IL-6 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-IL-6 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-IL-6 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-IL-6 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-IL-6 Magnetic Beads-IP Kit Product Description

The Anti-IL-6 magnetic Beads, conjugated with Anti-IL-6 antibody, are used for immuneprecipitation (IP) of IL-6 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing IL-6 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound IL-6 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-IL-6 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-IL-6 Antibody(10395-T52)
Immunogen
Recombinant Human IL6 / IL-6 protein (Catalog#10395-HNAE)
Species Reactivity
Human IL6 / IL-6
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human IL6 / IL-6 (rh IL6 / IL-6; Catalog#10395-HNAE; NP_000591.1; Val30-Met212). IL6 / IL-6 specific IgG was purified by Human IL6 / IL-6 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-IL-6 Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-BSF2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-HGFALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-HSFALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-IFNB2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-IL-6ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Interleukin-6ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

IL-6 Background Information

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 13 (gp13) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
Full Name
interleukin 6
Related Pathways
  • IL17 signaling pathway
    IL17 signaling pathway
References
  • Heinrich PC. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20.
  • Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24.
  • Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.
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