Anti-LAMP1 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-LAMP1 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-LAMP1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-LAMP1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-LAMP1 Magnetic Beads-IP Kit Product Description

The Anti-LAMP1 magnetic Beads, conjugated with Anti-LAMP1 antibody, are used for immuneprecipitation (IP) of LAMP1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing LAMP1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound LAMP1 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-LAMP1 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-LAMP1 Antibody(11215-RP02)
Immunogen
Recombinant Human LAMP1 protein (Catalog#11215-H08H)
Species Reactivity
Human LAMP1 / CD107a
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human LAMP1 extracellular domain (rh LAMP1; Catalog#11215-H08H; NP_005552.3; Met 1-Met 382). LAMP1 specific IgG was purified by human LAMP1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-LAMP1 Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-CD107aALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-LAMPAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-LGP120ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

LAMP1 Background Information

Lysosome-associated membrane glycoprotein 1, also known as CD17 antigen-like family member A, CD17a, and LAMP1, is a single-pass type I membrane protein which belongs to the LAMP family. CD17a is expressed largely in the endosome-lysosome membranes of cells, but is also found on the plasma membrane (1-2% of total LAMP1). LAMP1 has been implicated in a variety of cellular functions, including cancer metastasis. It has been proposed LAMP1 serves as a therapeutic agent for some cancers, as well as a marker for lysosomal storage disorders and different cell types such as cytotoxic T cells. LAMP2, also known as CD17b, may also play a role in tumor cell metastasis and functions in the protection, maintenance, and adhesion of the lysosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation. LAMP-1 is a glycoprotein highly expressed in lysosomal membranes. The present study was initiated to test LAMP-1 mRNA and protein levels in post mortem frontal cortex (area 8) of Alzheimer's disease (AD) stages I-IIA/B and stages V-VIC of Braak and Braak, compared with age-matched controls. LAMP-1 occurred in microglia and multinucleated giant cells in one AD case in whom amyloid burden was cleared following betaA-peptide immunization. In addition, LAMP-1 has been suggested to be a cell surface receptor for a specific amelogenin isoform, leucine-rich amelogenin peptide or LRAP. LAMP-1 can serve as a cell surface binding site for amelogenin on dental follicle cells and cementoblasts.
Full Name
lysosomal-associated membrane protein 1
References
  • Parkinson-Lawrence EJ, et al. (2005) Immunochemical analysis of CD107a (LAMP-1). Cell Immunol. 236(1-2): 161-6.
  • Barrachina M, et al. (2006) Lysosome-associated membrane protein 1 (LAMP-1) in Alzheimer's disease. Neuropathol Appl Neurobiol. 32(5): 505-16.
  • Zhang H, et al. (2010) Full length amelogenin binds to cell surface LAMP-1 on tooth root/periodontium associated cells. Arch Oral Biol. 55(6): 417-25.
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