Anti-MEK1 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-MEK1 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-MEK1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-MEK1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-MEK1 Magnetic Beads-IP Kit Product Description

The Anti-MEK1 magnetic Beads, conjugated with Anti-MEK1 antibody, are used for immuneprecipitation (IP) of MEK1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing MEK1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound MEK1 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-MEK1 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-MEK1 Antibody(101351-T38)
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human MEK1/MAP2K1/MKK1
Species Reactivity
Human Mouse, Rat (Species predicted to react based on 100% sequence homology)
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human MEK1/MAP2K1/MKK1, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-MEK1 Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-CFC3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MAPKK1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MEK1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MKK1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PRKMK1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

MEK1 Background Information

MEK1, also known as MAP2K1 and MKK1, is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MEK1 is widely expressed, with extremely low levels in brain. It lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Binding extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1 and MEK2. MEK1 has been shown to export PPARG from the nucleus. The MAPK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. MKK1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Defects in MEK1 can cause cardiofaciocutaneous syndrome.
Full Name
mitogen-activated protein kinase kinase 1
Research Areas
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • EGFR Signaling Pathway
    EGFR Signaling Pathway
  • TGF-beta Signaling
    TGF-beta Signaling
  • VEGF Signaling Pathway
    VEGF Signaling Pathway
  • Actin Dynamics Signaling Pathway
    Actin Dynamics Signaling Pathway
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
  • G Protein-coupled Receptors Signaling
    G Protein-coupled Receptors Signaling
  • Jak-Stat Signaling Pathway
    Jak-Stat Signaling Pathway
  • MAPK-Erk Pathway
    MAPK-Erk Pathway
  • T Cell Receptor Signaling Pathway
    T Cell Receptor Signaling Pathway
  • Common G-Chain signaling pathway
    Common G-Chain signaling pathway
  • IL1 signaling pathway
    IL1 signaling pathway
  • IL17 signaling pathway
    IL17 signaling pathway
References
  • Rampoldi L, et al. (1998) Chromosomal localization of four MAPK signaling cascade genes: MEK1, MEK3, MEK4 and MEKK5. Cytogenet Cell Genet. 78(3-4):301-3.
  • Zheng CF, et al. (1993) Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J Biol Chem. 268(15):11435-9.
  • Nantel, et al. (1998) Interaction of the Grb10 adapter protein with the Raf1 and MEK1 kinases. J Biol Chem. 273(17):10475-84.
  • Hirata H, et al. (2012) I MicroRNA-1826 targets VEGFC, beta-catenin (CTNNB1) and MEK1 (MAP2K1) in human bladder cancer. Carcinogenesis. 33(1):41-8.
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