Anti-Human respiratory syncytial virus (RSV) Fusion Glycoprotein / RSV-F Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-RSV Fusion Magnetic Beads-IP Kit Product Components

Components Storage
Anti-RSV Fusion Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-RSV Fusion magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-RSV Fusion Magnetic Beads-IP Kit Product Description

The Anti-RSV Fusion magnetic Beads, conjugated with Anti-RSV Fusion antibody, are used for immuneprecipitation (IP) of RSV Fusion proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing RSV Fusion proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound RSV Fusion proteins are dissociated from the magnetic beads using an elution buffer.

Anti-RSV Fusion Magnetic Beads-IP Kit Antibody Information

Antibody
Human respiratory syncytial virus (RSV) Fusion Glycoprotein / RSV-F Antibody, Rabbit MAb(11049-R009)
Immunogen
Recombinant Human RSV Fusion Glycoprotein / RSV-F (Catalog#11049-V08B)
Species Reactivity
Human RSV Fusion Glycoprotein /RSV-F
Source
Monoclonal RSV Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human RSV fusion glycoprotein (Catalog#11049-V08B; AAB59858.1; Met 1-Thr 529).
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-Human respiratory syncytial virus (RSV) Fusion Glycoprotein / RSV-F Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-FALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-HRSVgp08ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

RSV Fusion Background Information

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.
References
  • Martin-Gallardo A. et al., 1993, J Gen Virol. 74 (3): 453-8.
  • Jose A M. et al., 1997, J Gen Virol. 78: 2411-8.
  • Feldman SA. et al., 1999, J Virol. 73 (8): 6610-7.
  • Zlateva K.T. et al., 2004, J Virol. 78 (9): 4675-83.
  • Trento A. et al., 2006, J Virol. 80 (2): 975-84.
  • Branigan P J. et al., 2006, J Gen Virol. 87 (2): 395-8.
  • Eckardt-Michel J. et al., 2008, J. Virol. 82: 3236-49.
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