Anti-STAT6 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-STAT6 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-STAT6 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-STAT6 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-STAT6 Magnetic Beads-IP Kit Product Description

The Anti-STAT6 magnetic Beads, conjugated with Anti-STAT6 antibody, are used for immuneprecipitation (IP) of STAT6 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing STAT6 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound STAT6 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-STAT6 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-STAT6 Antibody(201061-T36)
Immunogen
E. coli-derived Human STAT6 fragment
Species Reactivity
Human STAT6
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with E. coli-derived Human STAT6 fragment, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-STAT6 Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-D12S1644ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-IL-4-STATALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-STAT6BALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-STAT6CALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

STAT6 Background Information

Signal transducer and activator of transcription 6 (STAT6) is a transcription factor that is activated by interleukin-4 (IL-4)-induced tyrosine phosphorylation and mediates most of the IL-4-induced gene expression. STAT6 plays a central role in exerting interleukin-4 (IL-4) mediated biological responses and is found to induce the expression of BCL2L1/BCL-XL, which is responsible for the anti-apoptotic activity of IL4. Transcriptional activation by STAT6 requires the interaction with coactivators like p3 and the CREB-binding protein (CBP). NF-?B and tyrosine-phosphorylated Stat6 can directly bind each other in vitro and in vivo, which suggest that the direct interaction between Stat6 and NF-?B may provide a basis for synergistic activation of transcription by IL-4 and activators of NF-?B.
Full Name
signal transducer and activator of transcription 6, interleukin-4 induced
Related Pathways
  • Jak-Stat Signaling Pathway
    Jak-Stat Signaling Pathway
  • Common G-Chain signaling pathway
    Common G-Chain signaling pathway
References
  • Litterst CM, et al. (2001) Transcriptional activation by STAT6 requires the direct interaction with NCoA-1. J Biol Chem. 276 (49): 45713-21.
  • Stutz AM, et al. (1999) Functional synergism of STAT6 with either NF-kappa B or PU.1 to mediate IL-4-induced activation of IgE germline gene transcription. J Immunol. 163 (8): 4383-91.
  • Yang J, et al. (2002) Identification of p100 as a coactivator for STAT6 that bridges STAT6 with RNA polymerase II. EMBO J. 21 (18): 4950-8.
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