One-hour, microfluidic SARS-CoV-2 antibody immunoassay using Sino Biological reagents and Gyrolab® immunoassay platform

The ongoing COVID-19 pandemic has triggered an unprecedented response from the biomedical community to develop rapid diagnostic tests, vaccines, and therapies against the SARS-CoV-2 virus. This has created an urgent need for innovative solutions for bioanalysis for serologic assays in ongoing community surveillance efforts, and to support vaccine development. To meet this need, Gyros Protein Technologies and Sino Biological have joined together to demonstrate how high-quality reagents from Sino Biological can be used in a high-performance, one-hour microfluidic, nanoliterscale immunoassay using Gyrolab® platform for the qualitative detection of total antibodies generated against the receptor binding domain (RBD) of the spike protein of SARS-CoV-2 in human serum samples.

Sino Biological, supplier for recombinant antigens and antibodies with a large collection of antibodies against different antigens of SARS-CoV-2 and recombinant forms of target SARS-CoV-2 proteins required for assay development, has supplied the high-quality reagents for the development of an anti-RBD SARS-CoV-2 antibody immunoassay. Gyros Protein Technologies, a provider of immunoassay bioanalytical tools, utilized Gyrolab miniaturized immunoassay platform and a microfluidic, flow-through affinity column in a CD format to develop this automated Gyrolab immunoassay for 112 datapoints per CD.

Sino Biologicals and Gyros Protein Technologies reagents and labware used in Gyrolab SARS-CoV-2 antibody serology assay

Reagent/ consumable Description Supplier Reagent preparation
Capture antigen Spike RBD-His Recombinant Protein, Biotinylated BSA Sino Biological, 40592-V08H 70 nM biotinylated Spike RBD-His Recombinant Protein + 630 nM biotinylated BSA in PBS-T
Vector Laboratories, B-2007
Detection antibody Spike RBD-Fc Recombinant Protein, Rexxip F Sino Biological, 40592-V02H Alexa Fluor 647-labeled Spike RBD-Fc Recombinant Protein, 20 nM in Rexxip F 
Gyros Protein Technologies, P0004825
Positive control antibody  SARS-CoV-2 Spike RBD rabbit polyclonal antibody Rexxip H Sino Biological, 40592-T62 Spike RBD rabbit polyclonal antibody in Rexxip H with 25% human serum 
Gyros Protein Technologies, P0004822
CD type  Bioaffy 200 CD Gyros Protein Technologies, P0004180 Bioaffy 200 CD 
Wash buffer for needles  Gyrolab Wash Buffer pH 11 Gyros Protein Technologies, P0020096 Wash buffer 1: PBS-T 
Wash buffer 2: Gyrolab Wash Buffer pH 11 
Sample        4 μL (sample diluted 1:4)
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