Anti-NGF Antibody

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Anti-NGF Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-NGF Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human NGF
Immunogen
Recombinant Human NGF/NGFB/beta-NGF Protein (Catalog#11050-HNAC)
Preparation
Produced in rabbits immunized with purified, recombinant Human NGF/NGFB/beta-NGF (rh NGF/NGFB/beta-NGF; Catalog#11050-HNAC; NP_002497.2; Ser122-Arg239). NGF/NGFB/beta-NGF specific IgG was purified by Human NGF/NGFB/beta-NGF affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-NGF Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:25000-1:50000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-NGF Antibody (Rabbit Polyclonal antibody) Images

Anti-β-NGF rabbit polyclonal antibody at 1:500 dilution

Lane A: 293 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:27 kDa

Observed band size:27 kDa

Anti-NGF Antibody: Alternative Names

Anti-Beta-NGF Antibody; Anti-HSAN5 Antibody; Anti-NGFB Antibody

NGF Background Information

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.
Full Name
nerve growth factor (beta polypeptide)
References
  • Castellanos MR, et al. (2003) Evaluation of the neurorestorative effects of the murine beta-nerve growth factor infusions in old rat with cognitive deficit. Biochem Biophys Res Commun. 312(4): 867-72.
  • Wang TH, et al. (2008) Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson's disease. J Mol Neurosci. 35(2): 161-9.
  • Perrard MH, et al. (2009) Redundancy of the effect of TGFbeta1 and beta-NGF on the second meiotic division of rat spermatocytes. Microsc Res Tech. 72(8): 596-602.
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