CCL5/RANTES Matched ELISA Antibody Pair Set,Human

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CCL5/RANTES Matched ELISA Antibody Pair Set,Human General Information

Product name
CCL5/RANTES Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human CCL5/RANTES
Assay range
0.78-50 pg/mL
Materials provided
1. Capture Antibody 1 mg/mL of mouse anti-Human CCL5 / RANTES monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10900-MM42)
2. Detection Antibody 0.2 mg/mL of mouse anti-Human CCL5 / RANTES monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in Dilution Buffer before use. (Catalog: # 10900-MM05)
3. Standard Each vial contains 9 ng of recombinant Human CCL5 / RANTES. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 50 pg/mL is recommended.
Product overview
This CCL5/RANTES Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human CCL5/RANTES . It contains Human CCL5/RANTES capture antibody, Human CCL5/RANTES detector antibody (HRP) and a highly purified recombinant Human CCL5/RANTES protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

CCL5/RANTES Matched ELISA Antibody Pair Set,Human Images

CCL5/RANTES Matched ELISA Antibody Pair Set,Human: Alternative Names

D17S136E Matched ELISA Antibody Pair Set, Human; eoCP Matched ELISA Antibody Pair Set, Human; RANTES Matched ELISA Antibody Pair Set, Human; SCYA5 Matched ELISA Antibody Pair Set, Human; SIS-delta Matched ELISA Antibody Pair Set, Human; SISd Matched ELISA Antibody Pair Set, Human; TCP228 Matched ELISA Antibody Pair Set, Human

CCL5/RANTES Background Information

Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokin ligand 5(CCL5) is chemotactic for T cells, basophils and eosinophils. Chemokin ligand 5(CCL5) has been considered a HIV-supressor secreted by CD8+ T cells and other immune cells. Chemokin ligand 5(CCL5) is a key to activating recruit leukocytes into inflammatory sites and in the presence of particular cytokines released by T cells, it can change the NK cells into CHAK cells.
Full Name
chemokine (C-C motif) ligand 5
References
  • Laing KJ, et al. (2004) Chemokines. Developmental and comparative immunology. 28(5): 443-60.
  • Cocchi F, et al. (1995) Identification of RANTES, MIP-1a, and MIP-1b as the major HIV-suppressive factor produced by CD8+ T cells. Science. 270 (5243): 1811-5.
  • Vangelista L, et al. (2010) Engineering of Lactobacillus jensenii to secrete RANTES and a CCR5 antagonist analogue as live HIV-1 blockers. Antimicrob. Agents Chemother. 54 (7): 2994-3001.
  • Maghazachi AA, et al. (1996) CC chemokines induce the generation of killer cells from CD56+ cells. Eur. J. Immunol. 26 (2): 315-9.
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