|Vector Type||Mammalian Expression Vector|
|Expression Method||Constitutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
|Sequencing Primer||Forward: T7(TAATACGACTCACTATAGGG)； Reverse: BGH(TAGAAGGCACAGTCGAGG)|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.