Influenza A H1N1 Nucleoprotein / NP Protein (His Tag)

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Influenza A H1N1 Nucleoprotein / NP Protein (His Tag): Product Information

Purity
> 97 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) nucleoprotein (AAM75159.1) (Met1-Gly490) was fused with a polyhistidine tag at the C-terminus.
Accession#
Expressed Host
Baculovirus-Insect Cells
Species
H1N1
Predicted N Terminal
Met 1
Molecule Mass
The recombinant Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) nucleoprotein comprises 501 amino acids and has a predicted molecular mass of 56.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 60mM Tris, 500mM NaCl, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Influenza A H1N1 Nucleoprotein / NP Protein (His Tag): Images

Influenza A H1N1 Nucleoprotein / NP Protein (His Tag): Synonyms

NP Protein, H1N1

Nucleocapsid/NP Background Information

Influenza A viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation.The influenza A virus nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs from all strains of influenza A viruses contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite.
  • CONSTRUCTION OF RECOMBINANT ADENOVIRAL VECTOR EXPRESSING GENES OF THE CONSERVATIVE INFLUENZA PROTEINS M2 AND NUCLEOPROTEIN
    Author
    ИБ Есмагамбетов;ЕС Седова;
    Year
    2014
    Journal
    Молекулярная генетика, микробиология и вирусология
    Application
    ELISA
  • Evaluating the potential of novel virus-vectored vaccines as intranasal vaccine candidates for broad immunity against influenza
    Author
    Mullin, J;Ahmed, MS;Upile, N;Vaughan, C;
    Year
    2014
    Journal
    University of Liverpool
  • Whole-Virus Screening to Develop Synbodies for the Influenza Virus
    Author
    Gupta, N;Lainson, J;Domenyuk, V;Zhao, ZG;Johnston, SA;Diehnelt, CW;
    Year
    2016
    Journal
    Bioconjug. Chem.
    Application
    SPR
  • Influenza Virus Vaccination Elicits Poorly Adapted B Cell Responses in Elderly Individuals
    Author
    Henry, C;Zheng, NY;Huang, M;Cabanov, A;Rojas, KT;Kaur, K;Andrews, SF;Palm, AE;Chen, YQ;Li, Y;Hoskova, K;Utset, HA;Vieira, MC;Wrammert, J;Ahmed, R;Holden-Wiltse, J;Topham, DJ;Treanor, JJ;Ertl, HC;Schmader, KE;Cobey, S;Krammer, F;Hensley, SE;Greenberg, H;He, XS;Wilson, PC;
    Year
    2019
    Journal
    Cell Host Microbe
    Application
    ELISA
  • Gold nanopillar array with sharp surface plasmon resonances and the application in immunoassay
    Author
    Yanagawa, H;Hinamoto, T;Kanno, T;Sugimoto, H;Shioi, M;Fujii, M;
    Year
    2019
    Journal
    Journal of Applied Physics
    Application
    SPR
  • Tissue-resident CD4+ T helper cells assist protective respiratory mucosal B and CD8+ T cell memory responses
    Author
    Son, YM;Cheon, IS;Wu, Y;Li, C;Wang, Z;Chen, Y;
    Year
    2020
    Journal
    bioRxiv
    Application
    binding
  • Redefining CD4 T cell residency: Helper T cells orchestrate protective humoral immunity in the lung
    Author
    Swarnalekha, N;Schreiner, D;Litzler, LC;Iftikhar, S;
    Year
    2020
    Journal
    bioRxiv
    Application
    ELISpot
  • Magnetic Particle Spectroscopy for Detection of Influenza A Virus Subtype H1N1
    Author
    Wu, K;Liu, J;Saha, R;Su, D;Krishna, VD;Cheeran, MC;Wang, JP;
    Year
    2020
    Journal
    ACS Appl Mater Interfaces
    Application
    binding
  • Highly sensitive and universal detection strategy based on a colorimetric assay using target-specific heterogeneous sandwich DNA aptamer
    Author
    Kang, J;Yeom, G;Jang, H;Park, C;Kim, M;
    Year
    2020
    Journal
    Analytica Chimica Acta
    Application
    Elisa
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