Influenza A H1N1 (A/California/07/2009) Nucleoprotein / NP Protein (His Tag)

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Influenza A H1N1 (A/California/07/2009) Nucleoprotein / NP Protein (His Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the Influenza A virus (A/California/07/09(H1N1)) nucleoprotein (ACS94534.1) (Met1-Ser498) was expressed with a C-terminal polyhistidine tag.
Accession#
Expressed Host
Baculovirus-Insect Cells
Species
H1N1
Predicted N Terminal
Met
Molecule Mass
The recombinant nucleoprotein of Influenza A virus (A/California/07/09(H1N1)) comprises 509 amino acids and has a predicted molecular mass of 57.4 kDa. The apparent molecular mass of the protein is approximately 53.5 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Influenza A H1N1 (A/California/07/2009) Nucleoprotein / NP Protein (His Tag): Images

Influenza A H1N1 (A/California/07/2009) Nucleoprotein / NP Protein (His Tag): Synonyms

NP Protein, H1N1

Nucleocapsid/NP Background Information

Influenza A viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation.The influenza A virus nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs from all strains of influenza A viruses contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite.
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    Bose, ME;McCaul, KC;Mei, H;Sasman, A;He, J;Kramp, W;Shively, R;Yan, K;Henrickson, KJ;
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    Henrickson, SE;Manne, S;Dolfi, DV;Mansfield, KD;Parkhouse, K;Mistry, RD;Alpern, ER;Hensley, SE;Sullivan, KE;Coffin, SE;Wherry, EJ;
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