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CD10 Protein, Human, Recombinant (hFc Tag)

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CD10 Protein, Human, Recombinant (hFc Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH, (R&D Systems, Catalog # ES005) . The specific activity is >1000 pmoles/min/μg.
Protein Construction
A DNA sequence encoding the extracellular domain (Tyr 52-Trp 750) of human membrane metallo-endopeptidase (NP_000893.2) was fused with the Fc region of human IgG1 at the N-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Glu 20
Molecule Mass
The recombinant human MME/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 936 amino acids and predicts a molecular mass of 106 kDa. As a result of glycosylation, the rh Fc monomer migrates as approximately 120-130 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD10 Protein, Human, Recombinant (hFc Tag): Images

CD10 Protein, Human, Recombinant (hFc Tag): Alternative Names

CALLA Protein, Human; CD10 Protein, Human; NEP Protein, Human; SFE Protein, Human

CD10 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 1 (CD1), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD1 is a zinc-dependent metalloprotease enzyme that had function to degrade a number of small secreted peptides such as the amyloid beta peptide. It exist as a membrane-bound protein and have high concentration in kidney and lung tissues. Mutations in the CD1 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD1 is also associated with other biochemical processes.
Full Name
membrane metallo-endopeptidase
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12
  • Dogan, et al. (2000) CD10 and BCL-6 Expression in Paraffin Sections of Normal Lymphoid Tissue and B-Cell Lymphomas. American Journal of Surgical Pathology. 24(6): 846-52.
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