FGF10 Protein, Human, Recombinant

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FGF10 Protein, Human, Recombinant: Product Information

Purity
> 98 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. The ED50 for this effect is 20-120 ng/mL.
Protein Construction
A DNA sequence encoding the mature form of human FGF10 (NP_004456.1) (Gln38-Ser208) was expressed.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human FGF10 consists of 172 amino acids and has a predicted molecular mass of 19.5 kDa as estimated in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, 1mM DTT, pH7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FGF10 Protein, Human, Recombinant: Images

Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. The ED50 for this effect is 20-120 ng/mL.

Citation List

FGF10 Protein, Human, Recombinant: Synonyms

KGF2 Protein, Human

FGF10 Background Information

Fibroblast growth factor 10 (FGF10) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF10 exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. FGF10 is required for normal branching morphogenesis. It may play a role in wound healing. Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG). ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.
Full Name
fibroblast growth factor 10
References
  • Sekine K, et al. (1999) Fgf10 is essential for limb and lung formation. Nat Genet. 21(1): 138-41.
  • Ohuchi H, et al. (2000) FGF10 acts as a major ligand for FGF receptor 2 IIIb in mouse multi-organ development. Biochem Biophys Res Commun. 277(3): 643-9.
  • Bellusci S, et al. (1997) Fibroblast growth factor 10 (FGF10) and branching morphogenesis in the embryonic mouse lung. Development. 124(23): 4867-78.
  • Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification
    Author
    Jiang, K;Tang, Z;Li, J;Wang, F;Tang, N;
    Year
    2018
    Journal
    Sci Rep
    Application
    tissue-based
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