IL-35 Protein, Human, Recombinant (hFc Tag)

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IL-35 Protein, Human, Recombinant (hFc Tag): Product Information

Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human IL35 complex composed of IL27B subunit (NP_005746.2) (Met 1-Lys 229) and the mature form of human IL12 p35 subunit (P29459) (Arg 23-Ser 219) linked by a polypeptide linker was fused with the Fc region of human IgG1 at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Arg 21
Molecule Mass
The recombinant human IL35/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 645 amino acids and has a predicted molecular mass of 73.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL35/Fc monomer is approximately 90-100 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-35 Protein, Human, Recombinant (hFc Tag): Images

IL-35 Protein, Human, Recombinant (hFc Tag): Synonyms

CLMF Protein, Human; IL-12A Protein, Human; IL-35 Protein, Human; Interleukin-35 Protein, Human; NFSK Protein, Human; NKSF1 Protein, Human; P35 Protein, Human

IL-35 Background Information

IL12A is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. IL12A, together with IL27B, form a disulfide-linked heterodimer: IL12A&IL27B. IL12A&IL27B is required for the T-cell-independent induction of IFN-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of IL12A&IL27B in innate immunity.
Full Name
interleukin 12A
Related Pathways
  • IL12 signaling pathway
    IL12 signaling pathway
References
  • Wolf S.F., et al.,(1991), Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells. J. Immunol. 146:3074-3081.
  • Gubler U., et al., (1991), Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor.Proc. Natl. Acad. Sci. U.S.A. 88:4143-4147.
  • Batten M., et al.,(2007), The biology and therapeutic potential of interleukin 27.J. Mol. Med. 85:661-672.
  • Reduced IL-35 levels are associated with increased platelet aggregation and activation in patients with acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation
    Author
    Zhang, X;Zhou, Y;Xu, L;Han, W;Chen, H;Chen, Y;Fu, H;Zhou, S;Zhao, J;Wang, Q;Feng, F;Zhu, X;Liu, K;Huang, X;
    Year
    2014
    Journal
    Ann. Hematol.
    Application
    cell-based
  • Anti-inflammatory effects of interleukin-35 in acquired aplastic anemia
    Author
    Yu, W;Ge, M;Lu, S;Shi, J;Li, X;Zhang, J;Wang, M;Huang, J;Shao, Y;Huang, Z;Zhang, J;Nie, N;Zheng, Y;
    Year
    2015
    Journal
    Cytokine
    Application
    cell culture
  • IL-35 Is Involved in the Pathogenesis of Guillain-Barré Syndrome Through Its Influence on the Function of CD4+ T Cells
    Author
    Liu, H;Zhang, W;Tian, FF;Kun, A;Zhou, WB;Xiao, B;Li, J;
    Year
    2015
    Journal
    Immunol. Invest.
    Application
    cell-based
  • Interleukin 35 may contribute to the loss of immunological self-tolerance in patients with primary immune thrombocytopenia
    Author
    Sun, T;Zhang, D;Yang, Y;Zhang, X;Lv, C;Fu, R;Lv, M;Liu, W;Chen, Y;Liu, W;Huang, Y;Xue, F;Liu, X;Zhang, L;Li, H;Yang, R;
    Year
    2015
    Journal
    Br. J. Haematol.
    Application
    cell-based
  • IL-35 recombinant protein reverses inflammatory bowel disease and psoriasis through regulation of inflammatory cytokines and immune cells
    Author
    Wang, Y;Mao, Y;Zhang, J;Shi, G;Cheng, L;Lin, Y;Li, Y;Zhang, X;Zhang, Y;Chen, X;Deng, J;Su, X;Dai, L;Yang, Y;Zhang, S;Yu, D;Wei, Y;Deng, H;
    Year
    2017
    Journal
    J. Cell. Mol. Med.
    Application
    animal-based
  • Expression of IGF-1, IL-27 and IL-35 Receptors in Adjuvant Induced Rheumatoid Arthritis Model
    Author
    Abdi, E;Najafipour, H;Joukar, S;Dabiri, S;Esmaeli-Mahani, S;Abbasloo, E;Houshmandi, N;Afsharipour, A;
    Year
    2018
    Journal
    Iran J Immunol
    Application
    animal-based
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