IL-35 Protein, Human, Recombinant

1/1
Price:
Size:
Number:

IL-35 Protein, Human, Recombinant: Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the p35 subunit of human IL12, termed as IL12A (NP_000873.2) (Met1-Ser219) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human IL27B, (NP_005746.2) (Met1-Lys229) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Arg 23 & Arg 21
Molecule Mass
The recombinant human IL12A&IL27B comprises 905 (447+458) amino acids and has a calculated molecular mass of 102 (50.7+ 51.3) KDa. The apparent molecular mass of the recombinant human IL12A&IL27B is approximately 60 & 63 KDa respectively in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-35 Protein, Human, Recombinant: Images

IL-35 Protein, Human, Recombinant: Synonyms

CLMF Protein, Human; IL-12A Protein, Human; IL-35 Protein, Human; Interleukin-35 Protein, Human; NFSK Protein, Human; NKSF1 Protein, Human; P35 Protein, Human

IL-35 Background Information

IL12A is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. IL12A, together with IL27B, form a disulfide-linked heterodimer: IL12A&IL27B. IL12A&IL27B is required for the T-cell-independent induction of IFN-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of IL12A&IL27B in innate immunity.
Full Name
interleukin 12A
Related Pathways
  • IL12 signaling pathway
    IL12 signaling pathway
References
  • Wolf S.F., et al.,(1991), Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells. J. Immunol. 146:3074-3081.
  • Gubler U., et al., (1991), Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor.Proc. Natl. Acad. Sci. U.S.A. 88:4143-4147.
  • Batten M., et al.,(2007), The biology and therapeutic potential of interleukin 27.J. Mol. Med. 85:661-672.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.