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IL-7 Protein, Human, Recombinant

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IL-7 Protein, Human, Recombinant: Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
1. Measured by its binding ability in a functional ELISA.
Immobilized human IL7 at 10 μg/ml (100 μl/well) can bind human IL7Ra-Fch (Cat:10975-H03H), The EC50 of human IL7Ra-Fch (Cat:10975-H03H) is 15.2-35.6 ng/ml.
2. Measured in a cell proliferation assay using antibody against CD3-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is typically 1-10 ng/ml.
Protein Construction
A DNA sequence encoding the mature form of human IL7 (Asp26-His177) was expressed with a N-terminal Met.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human IL7 consists of 153 amino acids and predicts a molecular mass of 17.5 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 25mM NaH2PO4, 400mM NaCl, pH 6.8.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-7 Protein, Human, Recombinant: Images

Measured in a cell proliferation assay using antibody against CD3-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is typically 1-10 ng/ml.

IL-7 Protein, Human, Recombinant: Alternative Names

IL-7 Protein, Human; Interleukin-7 Protein, Human

IL-7 Background Information

IL7, also known as interleukin 7, is a hematopoietic growth factor which belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes.
Full Name
interleukin 7
References
  • Watanabe M, et al. (1995) Interleukin 7 is produced by human intestinal epithelial cells and regulates the proliferation of intestinal mucosal lymphocytes.
  • J Clin Invest. 95(6):2945-53. Sawa Y, et al. (2009) Hepatic interleukin-7 expression regulates T cell responses. Immunity. 30 (3):447-57.
  • Flad HD, et al. (1996) Human follicular dendritic cells and vascular cells produce interleukin-7: a potential role for interleukin-7 in the germinal center reaction. Eur J Immunol. 26(10): 2541-4.
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