CTLA-4 Protein, Mouse, Recombinant (His Tag): Product Information
> 98 % as determined by SDS-PAGE
< 1.0 EU per μg of the protein as determined by the LAL method
1. Measured by its binding ability in a functional ELISA. 2. Immobilized recombinant mouse CTLA4-His (Cat:50503-M08H) at 10 μg/ml (100 μl/well) can mouse CD80-Fc (cat:50446-M02H) with a linear range of 0.78-62.5 ng/ml. 3. Immobilized recombinant mouse CTLA4-His (Cat:50503-M08H) at 10 μg/ml (100 μl/well) can human B7-1-Fc (Cat:10698-H03H) with a linear range of 0.78-125 ng/ml. 4. Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.5μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80.
A DNA sequence encoding the extracellular domain of mouse CTLA4 (NP_033973.2) (Met 1-Phe 162) was expressed, with a polyhistidine tag at the C-terminus.
The recombinant mouse CTLA4 consists of 138 amino acids and has a predicted molecular mass of 15.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CTLA4 is approximately 25-30 kDa due to glycosylation.
Lyophilized from sterile PBS, pH 7.4 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.5μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80.
Measured by its binding ability in a functional ELISA.
1. Immobilized recombinant mouse CTLA4-His (Cat: 50503-M08H) at 10 μg/ml (100 μl/well) can mouse CD80-Fc (Cat: 50446-M02H) with a linear range of 0.78-62.5 ng/ml.
2. Immobilized recombinant mouse CTLA4-His (Cat: 50503-M08H) at 10 μg/ml (100 μl/well) can human B7-1-Fc (Cat: 10698-H03H) with a linear range of 0.78-125 ng/ml.
Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD8 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).
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