Human Leukocyte Differentiation Antigen workshop Ⅰ/ HLDA1

In November 1982, the results of the First International Workshop on Human Leucocyte Differentiation Antigens, were presented at a meeting held in Paris. The data from these common blind studies were collected by 55 research groups from 14 different countries for publication. A total of 139 monoclonal antibodies were examined in immunofluorescence tests and the results expressed as a percentage of positive cells. The statistical analysis relied on the distances between antibodies. The distance between 2 antibodies for a given target cell type is the average of the absolute difference between the reactivities of the 2 antibodies for all target cells of the type considered. Then cluster analysis of the matrix of distances between all pairs of antibodies and algorithmic computation permitted the definition of groups of antibodies that were similar, and those that were clearly different, on each type of target cell.

The clusters of differentiation were then readily delineated from summing-up tables of groups of anti-bodies obtained for each target cell type; these clusters included the antibodies that always had very similar reactivities. Moreover, the confrontation between blind studies on the relative molecular mass ("molecular weight"), performed with the antibodies, and cluster analysis showed an almost perfect correspondence. These final clusters were termed clusters ofdifferentiation (CD) following the general recommendations adopted by the Nomenclature Subcommittee, which were approved with no objections or comments from the 250 scientists (including most of the workshop participants) at the Paris meeting mentioned above.

Clusters of differentiation of human leukocyte differentiation antigens

Name Alternative Name Function
CD1 Antigen presenting protein
CD2 T11, LFA-2, SRBC-R, Erythrocyte receptor, LFA-3R Adhesion between T-cells and other cell types and T-cell activation.
CD3 T-cell activation signaling and regulation of TCR expression
CD4 T4 T-cell activation,  thymic differentiation and receptor for HIV
CD5 T1, Tp67, LEU1 Regulates T-cell receptor signaling
CD6 T12, OX52, TP120 T-cell activation and cell adhesion
CD7 LEU 9, GP40, TP41 T-cell interactions
CD8 T8, Leu-2, MAL,CD8 alpha Co-receptor for MHC class 1 molecules
CD9 p24, MRP-1, 5H9 antigen, MIC3, TSPAN29, GIG2 Platelet activation and aggregation, and cell adhesion and cell motility
CD10 CALLA, NEP, gp100, Neprilysin, SFE, MME, EPN Peptidase, regulates B-cell growth
CD11 p150, 95, CR4, integrin αX, LFA-1A, ITGAX Cell adhesion
CDw13 Aminopeptidase N, APN, ANPEP,PEPN Aminopeptidase
CDw14 LPS-R Receptor for complex of LPS and LBP