Human Leukocyte Differentiation Antigen workshop Ⅶ / HLDA7

What is the aims and approaches of HLDA7?

The Human Leukocyte Differentiation Antigen workshop (HLDA7) took place in 2000 in Harrogate, United Kingdom, and the proceedings of the meeting (Leucocyte Typing VII1) have recently been published. Antibodies to the most immunogenic molecules had already been produced, and fewer laboratories than in the early days were prepared to devote resources to raising new antibodies, since the probability of finding novel reagents becomes ever less likely. In consequence many antibodies in the sixth workshop were reagents that proved to be of known specificity.

With these considerations in mind, HLDA7 adopted a different approach. Instead of screening poorly characterized antibodies, reagents were selected for which at least some molecular data were already available. A substantial number of monoclonal antibodies reactive with leukocyte-associated molecules exist that do not meet the traditional criterion for establishing a new CD specificity. Consequently, it is now considered appropriate to establish a CD designation for a molecule if its gene has been cloned and at least one specific monoclonal antibody has been studied in the workshop. Four new domains were introduced in HLDA7 to add to the traditional list from past meetings: Dendritic Cells, Stem/Progenitor Cells, Erythroid Cells, and Carbohydrate Structures.

Which is the yield of new CD antigens in HLDA7 ?

HLDA7 were added over 80 new molecules to the list of CD antigens. This compares favorably with previous workshops (an average of fewer than 30 CD specificities per workshop), and it also largely avoided the laborious screening in multiple laboratories of antibodies that often prove to be directed against known CD molecules.

New CD antigens list of HLDA10

New CD Alias Function
CD75 ST6GAL1, Sialyltransferase 1 Functional maturation of B lymphocytes
CD111 PVRL1, PRR1, Nectin-1, HVEC Entry mediator for several herpes viruses (HSV1, HSV2, PRV, BHV1).Believed to be involved in cell adhesion mechanisms
CD112 PVRL2, PRR2, Nectin-2, HVEB Intracellular adhesion, component of adherins junctions. Receptor for herpes simplex virus
CD156b ADAM17, ADAM18, CSVP, NISBD, NISBD1, TACE Cleaves transmembrane form of TNF-α to generate the soluble active form.
CD159a NKG2A, KLRC1, NKG2 Negative regulation of NK cell activation
CD160 BY55, NK1 Broad specificity receptor for MHC class I molecules
CD167a DDR1, MCK10 Receptor tyrosine kinase involved in cell-cell interactions
CD170 Siglec-5, CD33-like2, SIGLEC5, OB-BP2 Adhesion molecule mediating sialic-acid dependent binding to cells
CD171 NCAM-L1, L1CAM Multidomain cell adhesion molecule required for normal neurohistogenesis
CD172a SIRPα, SHPS-1, SIRPA Immunoglobulin-like cell surface receptor for CD47. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment.
CD176 Thomson Friedrenreich Ag, TRF1, TRF Potentially involved in cell adhesion
CD177 NB1 glycoprotein, HNA-2a Major immunogenic molecule of neutrophil membrane
CD178 FasL, CD95L, FASLG, Fas Ligand T-cell mediated cytotoxicity
CD179b IGLL1 Early B-cell differentiation.
CD180 RP-105 Controls B-cell recognition and signaling of LPS. Leads to NFkB activation.
CD183 CXCR3 T-cell chemotaxis, integrin activation, and adhesion
CD200 OX-2, OX2 Down-regulatory signal for myeloid cell function, costimulates T-cell proliferation
CD201 EPC-R, PROCR Enhances protein C activation
CD202b Tie2, Tek Signaling, migration and adhesion
CD204 Macrophage scavenger-R, MSR1 Endocytosis of macromolecules including low density lipoproteins, host defense
CD205 DEC-205, LY75 Phagocytosis, endocytosis
CD207 Langerin Pathogen receptor and processing of antigen for presentation to T-cells
CD208 DC-LAMP, LAMP3 Dendritic cell maturation marker, involved in cell proliferation
CD209 DC-SIGN Mediates antigen endocytosis and degradation binds pathogenic antigens such as HIV1 gp120
CD212 IL-12-R β1, IL12RB1 Forms part of the receptor complexes for IL12 and IL23
CD213a1 IL-13-R α1, IL13RA1 Forms part of the receptor complexes for IL13 and IL4
CD213a2 IL-13-R α2, IL13RA2 Forms part of the receptor complex for IL13.
CD220 Insulin-R, Insulin Receptor, IINSR Insulin receptor
CD221 IGF-1 R, IGF1R Receptor for IGF-I and IGF-II. Mediates mitogenic and anti-apoptotic signals
CD223 LAG-3 Involved in lymphocyte activation, negative regulation of T-cell activation
CD224 GGT1 Catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors, which contributes to oxidative homeostasis.
CD225 IFITM1, Leu-13, DSPA2a Cell adhesion, cell growth and migration. Relays antiproliferative and homotypic adhesion signals
CD226 DNAM-1, PTA-1, TLiSA1 Intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells
CD227 MUC1, EMA, PUM, Mucin 1 Cell adhesion and signaling
CD228 Melanotransferrin, MFI2 Involved in cellular iron uptake
CD229 Ly-9, LY9, SLAMF3 Adhesion between T-cells and accessory cells
CD230 Prion protein, PRNP Oxidative stress homeostasis,cell survival, and signal transduction
CD231 TALLA-1, A15, Tspan-7 Cell proliferation and motility
CD233 Band 3, SLC4A1 Anion exchanger across the cytoplasmic membrane, binding site for cytoskeletal proteins, enzymes and hemoglobin
CD235a Glycophorin A, GYPA Major erythrocyte intrinsic membrane protein. Minimizes erythrocyte aggregation, receptor for influenza virus, Plasmodium falciparum and Hepatitis A virus
CD236 Glycophorin C/D, GYPC Glycophorin-C regulates the stability of red cells
CD238 Kell, KEL, Blood Group Kell Zinc endopeptidase cleaving endothelin 3. Contains the Kell blood group antigens
CD239 B-CAM, BCAM Laminin alpha-5 receptor
CD242 ICAM-4 Ligand for the leukocyte adhesion protein LFA-1
CD244 2B4, NAIL NK cell activation, NK cell and T-cell interaction
CD247 TCRz, CD3-ZEETA Couples antigen recognition to several intracellular signaling pathways