Reagent Resource for Studying SARS-CoV-2 Variants

Several fast-spreading variants of SARS-CoV-2 virus have emerged in recent months: the U.K. variant B.1.1.7, the Brazil variant P.1, the South Africa variant B.1.351, and the India variant B.1.617.

A subset of the mutations identified in the RBD domain of the spike protein occurs in more than one strain, although the three variants are believed to be independently evolved. These convergent mutations, specifically, the N501Y (shared by all three, 40592-V08H82) and E484K (shared between B.1.351 and P.1, 40592-V08H84), are of high interest because they may be the cause of the increased transmissibility.

The mutations in these strains also occur in the nucleocapsid protein, which is commonly used as the biomarker in rapid antigen tests. It’s critical to assess whether the current commercial antigen tests can detect the mutated N proteins with the same sensitivity and specificity as their WT counterpart.

Sino Biological has developed a panel of recombinant RBD/Spike and nucleocapsid protein variants carrying the aforementioned mutations as below:

Recombinant Mutants from the India Variant  | B.1.617

This new variant, B.1.617, first indentified in India in October 2020 has emerged all around the world. This variant may be more transmissible and needs to be further investigated.

Catalogue Order Antigen Mutation
40592-V08H88 Order RBD L452R,E484Q
40592-V08H28 Order RBD L452R
40592-V08H81 Order RBD E484Q
40591-V08H19 under development S1 Spike D614G, Spike E154K, Spike E484Q, Spike Spike L452R, Spike P681R
40589-V08B12 under development S Spike D614G, Spike E154K, Spike E484Q, Spike E1072K, Spike K1073R, Spike L452R, Spike P681R
40589-V08B13 under development S Spike D614G, Spike E154K, Spike E484Q, Spike G142D, Spike H1101D, Spike L452R, Spike P681R, Spike Q1071H
40588-V07E20 under development N R203M,D377Y
40588-V07E16 under development N D377Y

Recombinant Mutants from the U.K. Variant  |  B.1.1.7

Catalogue Order Antigen Mutation
40592-V08H82 Order RBD N501Y
40592-V08H82-B Order RBD-Biotinylated N501Y
40591-V08H7 Order S1 HV69-70 deletion, N501Y, D614G
40591-V08H12 Order S1 H69del, V70del, Y144del, N501Y, A570D, D614G, P681H
40591-V08H12-B Order S1-Biotinylated H69del, V70del, Y144del, N501Y, A570D, D614G, P681H
40588-V07E7 Order N D3L, R203K, G204R, S235F
40588-V07E8 Order N D3L, S235F
40589-V08B6 Order S-ECD H69del, V70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
40592-V31H1 Order RBD N501Y

Recombinant Mutants from the South Africa Variant  |  B.1.351

Catalogue Order Antigen Mutation
40592-V08H82 Order RBD N501Y
40592-V08H82-B Order RBD-Biotinylated N501Y
40592-V08H84 Order RBD E484K
40592-V08H84-B Order RBD-Biotinylated E484K
40592-V08H59 Order RBD K417N
40592-V08H59-B Order RBD-Biotinylated K417N
40592-V08H85 Order RBD K417N, E484K, N501Y
40592-V08H85-B Order RBD-Biotinylated K417N, E484K, N501Y
40591-V08H10 Order S1 K417N, E484K, N501Y, D614G
40592-V31H2 Order RBD E484K
40592-V31H4 Order RBD K417N, E484K, N501Y
40592-V31H6 Order RBD K417N

Recombinant Mutants from the Brazil Variant  |  P.1

Catalogue Order Antigen Mutation
40592-V08H82 Order RBD N501Y
40592-V08H82-B Order RBD-Biotinylated N501Y
40592-V08H84 Order RBD E484K
40592-V08H84-B Order RBD-Biotinylated E484K
40592-V08H86 Order RBD K417T, E484K, N501Y
40589-V08B10 Order S1+S2 L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F
40592-V31H5 Order RBD K417T, E484K, N501Y
40592-V31H7 Order RBD K417T

Recombinant Mutants from the Denmark Mink Variant

Catalogue Order Antigen Mutation
40592-V08H80 Order RBD Y453F
40591-V08H8 Order S1 ΔH69/ΔV70, Y453F, D614G
40592-V31H3 Order RBD Y453F

High Frequency Mutation of Nucleocapsid

Catalogue Order Antigen Mutation
40588-V07E1 Order N R203K, G204R
40588-V07E2 Order N P13L
40588-V07E3 Order N S194L
40588-V07E4 Order N I292T
40588-V07E9 Order N T205I
40588-V07E11 Order N P80R

Conserved Domain of Nucleocapsid from SARS-CoV-2

Catalogue Order Antigen Domain
40588-V07E5 Order N-CTD CTD
40588-V07E10 Order N-NTD NTD

SARS-CoV-2 Nucleocapsid Mutant

  • Nucleocapsid His Tag
    P13L
    40588-V07E2
  • Nucleocapsid His Tag
    S194L
    40588-V07E3
  • Nucleocapsid His Tag
    R203K, G204R
    40588-V07E1
  • Nucleocapsid His Tag
    T205I
    40588-V07E9
  • Nucleocapsid His Tag
    I292T
    40588-V07E4
  • Nucleocapsid His Tag
    D3L, R203K, G204R, S235F
    40588-V07E7
  • Nucleocapsid His Tag
    D3L, S235F
    40588-V07E8
  • Nucleocapsid His Tag
    D377Y
    40588-V07E16
  • Nucleocapsid His Tag
    E378Q
    40588-V07E17
  • Nucleocapsid His Tag
    P80R
    40588-V07E11

SARS-CoV-2 S1+S2 ECD Mutant

SARS-CoV-2 Spike RBD Mutant

  • RBD His Tag
    P337S
    40592-V08H62
  • RBD His Tag
    F338L
    40592-V08H26
  • RBD His Tag
    V341I
    40592-V08H11
  • RBD His Tag
    F342L
    40592-V08H6
  • RBD His Tag
    A344S
    40592-V08H37
  • RBD His Tag
    A348S
    40592-V08H25
  • RBD His Tag
    A352S
    40592-V08H58
  • RBD His Tag
    N354D
    40592-V08H2
  • RBD His Tag
    S359N
    40592-V08H32
  • RBD mFc Tag
    V367F
    40592-V05H1
  • RBD His Tag
    V367F
    40592-V08H1
  • RBD His Tag
    N370S
    40592-V08H43
  • RBD His Tag
    A372S
    40592-V08H19
  • RBD His Tag
    A372T
    40592-V08H36
  • RBD His Tag
    F377L
    40592-V08H27
  • RBD His Tag
    K378N
    40592-V08H42
  • RBD His Tag
    K378R
    40592-V08H33
  • RBD His Tag
    P384L
    40592-V08H24
  • RBD His Tag
    T385A
    40592-V08H47
  • RBD His Tag
    T393P
    40592-V08H48
  • RBD His Tag
    V395I
    40592-V08H49
  • RBD His Tag
    D405V, Q414A
    40592-V08H22
  • RBD His Tag
    E406Q
    40592-V08H40
  • RBD His Tag
    R408I
    40592-V08H10
  • RBD His Tag
    Q409E
    40592-V08H34
  • RBD His Tag
    Q414E
    40592-V08H23
  • RBD His Tag
    Q414R
    40592-V08H44
  • RBD His Tag
    K417N
    40592-V08H59
  • RBD His Tag
    K417N
    40592-V08H59-B
  • RBD rFc Tag
    K417N
    40592-V31H6
  • RBD rFc Tag
    K417T
    40592-V31H7
  • RBD His Tag
    K417N, E484K, N501Y
    40592-V08H85
  • RBD His Tag
    K417N, E484K, N501Y
    40592-V08H85-B
  • RBD His Tag
    K417T, E484K, N501Y
    40592-V08H86
  • RBD rFc Tag
    K417T, E484K, N501Y
    40592-V31H4
  • RBD rFc Tag
    K417T, E484K, N501Y
    40592-V31H5
  • RBD His Tag
    A435S
    40592-V08H4
  • RBD His Tag
    W436R
    40592-V08H9
  • RBD His Tag
    N439K
    40592-V08H14
  • RBD His Tag
    N440K
    40592-V08H55
  • RBD His Tag
    K444R
    40592-V08H54
  • RBD His Tag
    V445F
    40592-V08H79
  • RBD His Tag
    G446S
    40592-V08H76
  • RBD His Tag
    G446V
    40592-V08H51
  • RBD His Tag
    L452R
    40592-V08H28
  • RBD His Tag
    L452R, E484Q
    40592-V08H88
  • RBD His Tag
    Y453F
    40592-V08H80
  • RBD rFc Tag
    Y453F
    40592-V31H3
  • RBD His Tag
    L455F
    40592-V08H68
  • RBD His Tag
    F456E
    40592-V08H73
  • RBD His Tag
    F456L
    40592-V08H71
  • RBD His Tag
    K458Q
    40592-V08H69
  • RBD His Tag
    K458R
    40592-V08H7
  • RBD His Tag
    E471Q
    40592-V08H56
  • RBD His Tag
    I472V
    40592-V08H35
  • RBD His Tag
    G476S
    40592-V08H8
  • RBD His Tag
    S477I
    40592-V08H45
  • RBD His Tag
    S477N
    40592-V08H46
  • RBD His Tag
    S477R
    40592-V08H64
  • RBD His Tag
    T478I
    40592-V08H30
  • RBD His Tag
    P479S
    40592-V08H57
  • RBD His Tag
    N481D
    40592-V08H70
  • RBD His Tag
    G482S
    40592-V08H53
  • RBD His Tag
    V483A
    40592-V08H5
  • RBD His Tag
    V483I
    40592-V08H31
  • RBD His Tag
    E484K
    40592-V08H84
  • RBD His Tag
    E484K
    40592-V08H84-B
  • RBD rFc Tag
    E484K
    40592-V31H2
  • RBD His Tag
    E484Q
    40592-V08H81
  • RBD His Tag
    G485S
    40592-V08H52
  • RBD His Tag
    F486A
    40592-V08H89
  • RBD His Tag
    F486S
    40592-V08H74
  • RBD His Tag
    N487R
    40592-V08H75
  • RBD His Tag
    F490L
    40592-V08H83
  • RBD His Tag
    F490S
    40592-V08H41
  • RBD His Tag
    S494P
    40592-V08H18
  • RBD His Tag
    P499R
    40592-V08H78
  • RBD His Tag
    N501Y
    40592-V08H82
  • RBD His Tag
    N501Y
    40592-V08H82-B
  • RBD Fc Tag
    N501Y
    40592-V02H1
  • RBD rFc Tag
    N501Y
    40592-V31H1
  • RBD His Tag
    V503F
    40592-V08H15
  • RBD His Tag
    Y505C
    40592-V08H72
  • RBD His Tag
    Y508H
    40592-V08H12
  • RBD His Tag
    A520S
    40592-V08H20
  • RBD His Tag
    A520V
    40592-V08H39
  • RBD His Tag
    P521R
    40592-V08H63
  • RBD His Tag
    P521S
    40592-V08H29
  • RBD His Tag
    A522S
    40592-V08H21
  • RBD His Tag
    A522V
    40592-V08H16

SARS-CoV-2 Spike S1 Mutant

SARS-CoV-2 Spike S1+S2 ECD Mutant

Recombinant Antibody Production