acnB Antibody Guideline: Preparation, Dilution and Applications

acnB Antibody Overview

There are 7 acnB antibodies which are validated in multiple tissues with various applications, including ELISA(Det), ELISA, WB, ELISA(Cap). There are 1 acnB antibody for ELISA(Det), 5 acnB antibody for ELISA, 2 acnB antibody for WB, 1 acnB antibody for ELISA(Cap). Among all these acnB antibodies, there are 4 anti-acnB mouse monoclonal antibodies , 1 anti-acnB rabbit monoclonal antibodies , 2 anti-acnB rabbit polyclonal antibodies . All the acnB anbodies are produced in house and all are in stock. acnB antibody customerized service is available.

acnB Antibody ELISA

acnB Antibody western blot

acnB Antibody western blot Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant E. coli acnB (Catalog#13102-ENAE; P36683; Met 1-Val 865). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

acnB Antibody western blot Validated

Application WB
Dilution his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect E.coli AcnB in WB. Using a DAB detection system, the detection limit for E.coli AcnB is approximately 4 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.