Alpha-fetoprotein Antibody Guideline: Preparation, Dilution and Applications

Alpha-fetoprotein Antibody Overview

There are 11 Alpha-fetoprotein antibodies which are validated in multiple tissues with various applications, including ELISA, ELISA(Det), ICC/IF, ELISA(Cap), FCM, WB, IP. There are 5 Alpha-fetoprotein antibody for ELISA, 1 Alpha-fetoprotein antibody for ELISA(Det), 1 Alpha-fetoprotein antibody for ICC/IF, 1 Alpha-fetoprotein antibody for ELISA(Cap), 3 Alpha-fetoprotein antibody for FCM, 2 Alpha-fetoprotein antibody for WB, 2 Alpha-fetoprotein antibody for IP. Among all these Alpha-fetoprotein antibodies, there are 8 anti-Alpha-fetoprotein mouse monoclonal antibodies , 1 anti-Alpha-fetoprotein rabbit monoclonal antibodies , 2 anti-Alpha-fetoprotein rabbit polyclonal antibodies . All the Alpha-fetoprotein anbodies are produced in house and all are in stock. Alpha-fetoprotein antibody customerized service is available.

Alpha-fetoprotein Antibody ELISA

Alpha-fetoprotein antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human AFP / alpha-fetoprotein (rh AFP / alpha-fetoprotein; Catalog#12177-H08H; P02771; Met 1-Val 609). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Alpha-fetoprotein antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

Alpha-fetoprotein Antibody western blot

Alpha-fetoprotein Antibody western blot Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human AFP / alpha-fetoprotein (rh AFP / alpha-fetoprotein; Catalog#12177-H08H; P02771; Met 1-Val 609). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Alpha-fetoprotein Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

Alpha-fetoprotein Antibody flow cytometry

Alpha-fetoprotein Antibody immunoprecipitation

Alpha-fetoprotein Antibody immunoprecipitation Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human AFP / alpha-fetoprotein (rh AFP / alpha-fetoprotein; Catalog#12177-H08H; P02771; Met1-Val609).

Alpha-fetoprotein Antibody immunoprecipitation Validated

Application IP
Dilution 0.5-2 μL/mg of lysate
Notes  

Alpha-fetoprotein Antibody ICC / IF

Alpha-fetoprotein Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human AFP / alpha-fetoprotein (rh AFP / alpha-fetoprotein; Catalog#12177-H08H; P02771; Met1-Val609). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Alpha-fetoprotein Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

Alpha-fetoprotein antibody citations

Title
Sensitive fluorescence immunoassay of alpha-fetoprotein through copper ions modulated growth of quantum dots in-situ
Year
2017
Author
Wang, Y;Chen, L;Liang, M;Xu, H;Tang, S;Yang, H;Song, H;
Journal
Sensors and Actuators B: Chemical
Title
Sensitive fluorescence immunoassay of alpha-fetoprotein through copper ions modulated growth of quantum dots in-situ
Year
2017
Author
Wang, Y;Chen, L;Liang, M;Xu, H;Tang, S;Yang, H;Song, H;
Journal
Sensors and Actuators B: Chemical