ASAM Antibody Guideline: Preparation, Dilution and Applications

ASAM Antibody Overview

There are 9 ASAM antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, WB. There are 8 ASAM antibody for ELISA, 2 ASAM antibody for FCM, 1 ASAM antibody for WB. Among all these ASAM antibodies, there are 2 anti-ASAM mouse monoclonal antibodies , 5 anti-ASAM rabbit polyclonal antibodies , 2 anti-ASAM rabbit monoclonal antibodies . All the ASAM anbodies are produced in house and all are in stock. ASAM antibody customerized service is available.

ASAM Antibody ELISA

ASAM antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human ASAM extracellular domain (rhASAM; Catalog#10794-H08H; NP_079045.1; Met 1-Met 233). Total IgG was purified by Protein A affinity chromatography.

ASAM antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

ASAM Antibody western blot

ASAM Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Mouse ASAM / CLMP (rM ASAM / CLMP; Catalog#50553-M08H; Q8R373-1; Met1-Met232). ASAM / CLMP specific IgG was purified by Mouse ASAM / CLMP affinity chromatography.

ASAM Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

ASAM Antibody flow cytometry

ASAM Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ASAM extracellular domain (rh ASAM; Catalog#10794-H08H; NP_079045.1; Met 1-Met 233). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

ASAM Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes