B7-H3 Antibody Guideline: Preparation, Dilution and Applications

B7-H3 Antibody Overview

There are 15 B7-H3 antibodies which are validated in multiple tissues with various applications, including ELISA, ELISA(Cap), ELISA(Det), FCM, ICC/IF, IP, IHC-P, WB. There are 10 B7-H3 antibody for ELISA, 1 B7-H3 antibody for ELISA(Cap), 1 B7-H3 antibody for ELISA(Det), 4 B7-H3 antibody for FCM, 1 B7-H3 antibody for ICC/IF, 3 B7-H3 antibody for IP, 2 B7-H3 antibody for IHC-P, 3 B7-H3 antibody for WB. Among all these B7-H3 antibodies, there are 6 anti-B7-H3 mouse monoclonal antibodies , 8 anti-B7-H3 rabbit polyclonal antibodies , 1 anti-B7-H3 rabbit monoclonal antibodies . All the B7-H3 anbodies are produced in house and all are in stock. B7-H3 antibody customerized service is available.

B7-H3 gene family antibody

B7-H3 Antibody ELISA

B7-H3 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human B7-H3/CD276 (rh B7-H3/CD276; Catalog#11188-H08H; Q5ZPR3-1; Met1-Thr461). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

B7-H3 antibody ELISA validated

Application ELISA
Dilution 1:5000-1:10000
Notes  

B7-H3 Antibody western blot

B7-H3 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Mouse B7-H3 / CD276 (Catalog#50973-M08H; NP_598744.1; Met1-Phe244). B7-H3 / CD276 specific IgG was purified by Mouse B7-H3 / CD276 affinity chromatography.

B7-H3 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

B7-H3 Antibody flow cytometry

B7-H3 Antibody immunohistochemistry

B7-H3 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human B7-H3/CD276 (rh B7-H3/CD276; Catalog#11188-H08H; Q5ZPR3-1; Met1-Thr461). B7-H3/CD276 specific IgG was purified by Human B7-H3/CD276 affinity chromatography.

B7-H3 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:2000-1:10000
Notes  

B7-H3 Antibody immunoprecipitation

B7-H3 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human B7-H3 / CD276 (rh B7-H3 / CD276; Catalog#11188-H08H; Q5ZPR3-1; Met 1-Thr 461). B7-H3 / CD276 specific IgG was purified by Human B7-H3 / CD276 affinity chromatography.

B7-H3 Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes  

B7-H3 Antibody ICC / IF

B7-H3 Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human B7-H3 / CD276 (rh B7-H3 / CD276; Catalog#11188-H08H; Q5ZPR3-1; Met 1-Thr 461). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

B7-H3 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:50-1:1000
Notes