BACE1 Antibody Guideline: Preparation, Dilution and Applications

BACE1 Antibody Overview

There are 8 BACE1 antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, ICC/IF, WB, IHC-P. There are 5 BACE1 antibody for ELISA, 3 BACE1 antibody for FCM, 1 BACE1 antibody for ICC/IF, 1 BACE1 antibody for WB, 1 BACE1 antibody for IHC-P. Among all these BACE1 antibodies, there are 1 anti-BACE1 mouse monoclonal antibodies , 4 anti-BACE1 rabbit monoclonal antibodies , 3 anti-BACE1 rabbit polyclonal antibodies . All the BACE1 anbodies are produced in house and all are in stock. BACE1 antibody customerized service is available.

BACE1 Antibody ELISA

BACE1 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BACE1 extracellular domain (rhBACE1; Catalog#10064-H08H; Met 1-Thr 457; NP_036236.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

BACE1 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

BACE1 Antibody western blot

BACE1 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Mouse BACE1 (rM BACE1; Catalog#50002-M08H; NP_035922.4; Met1-Thr457). BACE1 specific IgG was purified by Mouse BACE1 affinity chromatography.

BACE1 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

BACE1 Antibody flow cytometry

BACE1 Antibody immunohistochemistry

BACE1 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Mouse BACE1 (rM BACE1; Catalog#50002-M08H; NP_035922.4; Met1-Thr457). BACE1 specific IgG was purified by Mouse BACE1 affinity chromatography.

BACE1 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

BACE1 Antibody ICC / IF

BACE1 Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human BACE1 (rh BACE1; Catalog#10064-H08H; NP_036236.1; Met1-Thr457).

BACE1 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes