Bcl-2 Antibody Guideline: Preparation, Dilution and Applications

Bcl-2 Antibody Overview

There are 13 Bcl-2 antibodies which are validated in multiple tissues with various applications, including WB, IHC-P, FCM, IP, ELISA(Cap), ELISA, ELISA(Det). There are 5 Bcl-2 antibody for WB, 2 Bcl-2 antibody for IHC-P, 5 Bcl-2 antibody for FCM, 3 Bcl-2 antibody for IP, 1 Bcl-2 antibody for ELISA(Cap), 2 Bcl-2 antibody for ELISA, 1 Bcl-2 antibody for ELISA(Det). Among all these Bcl-2 antibodies, there are 6 anti-Bcl-2 rabbit monoclonal antibodies , 3 anti-Bcl-2 rabbit polyclonal antibodies , 4 anti-Bcl-2 mouse monoclonal antibodies . All the Bcl-2 anbodies are produced in house and all are in stock. Bcl-2 antibody customerized service is available.

Bcl-2 gene family antibody

Approved symbol Common name
BCL2L2 Bcl-W antibody
MKI67 Ki67/MKI67 antibody
PFKM PFKM antibody
Approved symbol Common name
BCL2A1 Bcl2A1 antibody
BCL2L12 BCL2L12 antibody
Approved symbol Common name
BCL2L1 Bcl-XL antibody
BCL2L2 Bcl-W antibody

Bcl-2 Antibody ELISA

Bcl-2 Antibody western blot

Bcl-2 Antibody western blot Preparation

This antibody was obtained from a rabbit immunized with purified, the synthetic peptide corresponding to the N-terminus of the Human Bcl-2.

Bcl-2 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

Bcl-2 Antibody flow cytometry

Bcl-2 Antibody flow cytometry Preparation

This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the N-terminus of the Human Bcl-2. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Bcl-2 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

Bcl-2 Antibody immunohistochemistry

Bcl-2 Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Bcl-2 (rh Bcl-2; Catalog#10195-H08E; P10415-1; Met1-Asp211). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Bcl-2 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:30-1:100
Notes  

Bcl-2 Antibody immunoprecipitation

Bcl-2 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, a synthetic peptide corresponding to the N-terminus of the Human Bcl-2. Human Bcl-2 specific IgG was purified by Human Bcl-2 affinity chromatography.

Bcl-2 Antibody immunoprecipitation Validated

Application IP
Dilution 1-2 μL/mg of lysate
Notes