Caspase-14 Protein Overview: Sequence, Structure, Function and Protein Interaction

Caspase-14 Protein Overview

Caspase-14 reagents

Using database searches for caspase sequences, Van de Craen et al. (1998) identified CASP14 in human genomic sequence data and cloned both human and mouse CASP14 cDNAs. By Northern blot and RT-PCR analyses, Van de Craen et al. (1998) detected Casp14 expression in mouse skin and developing mouse embryos (embryonic day 7, 15, 16, and 17). Expression generally increased during maturation of the mouse embryo but dropped temporarily to an undetectable level around embryonic day 11 to 12. The authors suggested that Casp14 may play a role in ontogenesis and skin physiology. Eckhart et al. (2000) cloned a full-length CASP14 cDNA encoding a 242-amino acid protein with potential AP1 (165160) and KLF4 (602253) interaction sites. They identified 2 CASP14 transcripts, designated CASP14a and CASP14b, that differ in the C terminus due to a shifted open reading frame. Use of an alternative splice acceptor site within intron 5 results in a 74-nucleotide insertion in CASP14b. CASP14b lacks homology with the caspase consensus sequence and does not have a counterpart in mouse. In situ hybridization analysis detected CASP14 within the epidermis, in hair follicles, and in the cells of the sebaceous glands, with highest expression in the keratogenous zone and the inner root sheeth of the hair follicle and in the peripheral cells of the sebaceous glands. Using immunohistochemical analysis, Denecker et al. (2007) confirmed that mouse Casp14 was expressed only in cornifying epithelia, such as the epidermis and Hassall bodies, and in forestomach, which is cornified like epidermis.

Caspase-14 protein family

Belongs to the peptidase C14A family.

Caspase-14 protein name

Recommended name
Caspase-14
Short name
CASP-14
Aliases
apoptosis-related cysteine protease, MGC119078, MGC119079, MICE
Alternative name
Caspase-14 subunit p17, mature form Caspase-14 subunit p10, mature form Caspase-14 subunit p20, intermediate form Caspase-14 subunit p8, intermediate form

Caspase-14 Gene family protein

Caspases

Caspase-14 Protein Sequence

Species Human Caspase-14 protein
Length 242
Mass (Da) 27680
Sequence Human Caspase-14 protein sequence
Species Mouse Caspase-14 protein
Length 257
Mass (Da) 29458
Sequence Mouse Caspase-14 protein sequence
Species
Length
Mass (Da)
Sequence

Caspase-14 Protein Molecular Weight & PI

Caspase-14 precursor (EC 3.4.22.-) (CASP-14) [Contains: Caspase-14 subunit p17, mature form; Caspase-14 subunit p10, mature form; Caspase-14 subunit p20, intermediate form; Caspase-14 subunit p8, intermediate form] Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 6-146 Caspase-14 subunit p17, mature form.

Molecular weight (Da)

16002.03

Theoretical pI

4.76

Caspase-14 Protein Interaction

Recombinant Caspase-14 Protein Feature

Caspase-14 Protein, Human, Recombinant (His Tag)

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
Please contact us for more information.
High Activity
Measured by its ability to bind biotinylated Cynomolgus IL18 (Cat:90011-C07E) in a functional ELISA.

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